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dc.contributor.authorElies, Jacobo*
dc.contributor.authorScragg, J.L.*
dc.contributor.authorDallas, M.L.*
dc.contributor.authorHuang, D.*
dc.contributor.authorHuang, S.*
dc.contributor.authorBoyle, J.P.*
dc.contributor.authorGamper, N.*
dc.contributor.authorPeers, C.*
dc.date.accessioned2017-06-15T09:07:11Z
dc.date.available2017-06-15T09:07:11Z
dc.date.issued2015
dc.identifier.citationElies J, Scragg JL, Dallas ML et al (2015) Inhibition of T-type Ca2+ channels by hydrogen sulfide. In: Peers C, Kumar P, Wyatt C et al (Eds.) Arterial chemoreceptors in physiology and pathophysiology. London: Springer.en_US
dc.identifier.urihttp://hdl.handle.net/10454/12224
dc.descriptionNoen_US
dc.description.abstractT-type Ca2+ channels are a distinct family of low voltage-activated Ca2+ channels which serve many roles in different tissues. Several studies have implicated them, for example, in the adaptive responses to chronic hypoxia in the cardiovascular and endocrine systems. Hydrogen sulfide (H2S) was more recently discovered as an important signalling molecule involved in many functions, including O2 sensing. Since ion channels are emerging as an important family of target proteins for modulation by H2S, and both T-type Ca2+ channels and H2S are involved in cellular responses to hypoxia, we have investigated whether recombinant and native T-type Ca2+ channels are a target for modulation by H2S. Using patch-clamp electrophysiology, we demonstrate that the H2S donor, NaHS, selectively inhibits Cav3.2 T-type Ca2+ channels heterologously expressed in HEK293 cells, whilst Cav3.1 and Cav3.3 channels were unaffected. Sensitivity of Cav3.2 channels to H2S required the presence of the redox-sensitive extracellular residue H191, which is also required for tonic binding of Zn2+ to this channel. Chelation of Zn2+ using TPEN prevented channel inhibition by H2S. H2S also selectively inhibited native T-type channels (primarily Cav3.2) in sensory dorsal root ganglion neurons. Our data demonstrate a novel target for H2S regulation, the T-type Ca2+ channel Cav3.2. Results have important implications for the proposed pro-nociceptive effects of this gasotransmitter. Implications for the control of cellular responses to hypoxia await further study.en_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttps://link.springer.com/chapter/10.1007%2F978-3-319-18440-1_40en_US
dc.subjectHydrogen sulfide; T-type Ca2+ channel; HEK293 cells; Sensory neuron; Zinc; Patch clampen_US
dc.titleInhibition of T-type Ca2+ channels by hydrogen sulfideen_US
dc.status.refereedYesen_US
dc.typeBook chapteren_US
dc.type.versionNo full-text in the repositoryen_US


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