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dc.contributor.authorAhmed, Mohaned S.A.*
dc.contributor.authorBasheer, Haneen A.*
dc.contributor.authorAyuso, J.M.*
dc.contributor.authorAhmet, Djevdet S.*
dc.contributor.authorMazzini, Marco*
dc.contributor.authorPatel, Roshan*
dc.contributor.authorShnyder, Steven*
dc.contributor.authorVinader, Victoria*
dc.contributor.authorAfarinkia, Kamyar*
dc.date.accessioned2017-05-02T16:00:57Z
dc.date.available2017-05-02T16:00:57Z
dc.date.issued2017-04-21
dc.identifier.citationAhmed M, Basheer HA, Ayuso JM et al (2017) Agarose Spot as a Comparative Method for in situ Analysis of Simultaneous Chemotactic Responses to Multiple Chemokines. Scientific Reports. 7: 1075.en_US
dc.identifier.urihttp://hdl.handle.net/10454/11904
dc.descriptionYesen_US
dc.description.abstractWe describe a novel protocol to quantitatively and simultaneously compare the chemotactic responses of cells towards different chemokines. In this protocol, droplets of agarose gel containing different chemokines are applied onto the surface of a Petri dish, and then immersed under culture medium in which cells are suspended. As chemokine molecules diffuse away from the spot, a transient chemoattractant gradient is established across the spots. Cells expressing the corresponding cognate chemokine receptors migrate against this gradient by crawling under the agarose spots towards their centre. We show that this migration is chemokine-specific; meaning that only cells that express the cognate chemokine cell surface receptor, migrate under the spot containing its corresponding chemokine ligand. Furthermore, we show that migration under the agarose spot can be modulated by selective small molecule antagonists present in the cell culture medium.en_US
dc.language.isoenen_US
dc.rights© 2017 The Authors. This article is licensed under a Creative Commons CC-BY 4.0 International License.en_US
dc.subjectCancer modelsen_US
dc.subjectDrug screening
dc.subjectChemotactic migration
dc.subjectAgarose gel
dc.subjectChemokines
dc.titleAgarose Spot as a Comparative Method for in situ Analysis of Simultaneous Chemotactic Responses to Multiple Chemokinesen_US
dc.status.refereedYesen_US
dc.date.Accepted2017-03-20
dc.typeArticleen_US
dc.type.versionPublished versionen_US
dc.identifier.doihttps://doi.org/10.1038/s41598-017-00949-4
refterms.dateFOA2018-07-25T13:11:16Z


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