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    Impact of lgt mutation on lipoprotein biosynthesis and in vitro phenotypes of Streptococcus agalactiae

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    Publication date
    2009-05-01
    Author
    Bray, B.A.
    Sutcliffe, I.C.
    Harrington, Dean J.
    Keyword
    Neonatal disease; Streptococcus agalactiae; B Streptococcus; Prenatal screening; Antiobiotics; GBS; Pathogenecity
    Peer-Reviewed
    Yes
    
    Metadata
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    Abstract
    Although Streptococcus agalactiae, the group B Streptococcus, is a leading cause of invasive neonatal disease worldwide the molecular basis of its virulence is still poorly understood. To investigate the role of lipoproteins in the physiology and interaction of this pathogen with host cells, we generated a mutant S. agalactiae strain (A909DeltaLgt) deficient in the Lgt enzyme and thus unable to lipidate lipoprotein precursors (pro-lipoproteins). The loss of pro-lipoprotein lipidation did not affect the viability of S. agalactiae or its growth in several different media, including cation-depleted media. The processing of two well-characterized lipoproteins, but not a non-lipoprotein, was clearly shown to be aberrant in A909DeltaLgt. The mutant strain was shown to be more sensitive to oxidative stress in vitro although the molecular basis of this increased sensitivity was not apparent. The inactivation of Lgt also resulted in changes to the bacterial cell envelope, as demonstrated by reduced retention of both the group B carbohydrate and the polysaccharide capsule and a statistically significant reduction (P=0.0079) in A909DeltaLgt adherence to human endothelial cells of fetal origin. These data confirm that failure to process lipoproteins correctly has pleiotropic effects that may be of significance to S. agalactiae colonization and pathogenesis.
    URI
    http://hdl.handle.net/10454/11570
    Version
    No full-text in the repository
    Citation
    Bray BA, Harrington DJ and Sutcliffe IC (2009) Impact of lgt mutation on lipoprotein biosynthesis and in vitro phenotypes of Streptococcus agalactiae. Microbiology. 155(Pt 5): 1451-1458.
    Link to publisher’s version
    http://dx.doi.org/10.1099/mic.0.025213-0
    Type
    Article
    Collections
    Life Sciences Publications

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