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    Nano-encapsulation of a novel anti-Ran-GTPase peptide for blockade of regulator of chromosome condensation 1 (RCC1) function in MDA-MB-231 breast cancer cells

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    Publication date
    2017-04
    Author
    Haggag, Y.A.
    Matchett, K.B.
    Dakir, El-Habib
    Buchanan, P.
    Osman, M.A.
    Elgizawy, S.A.
    El-Tanani, Mohamed
    Faheem, A.M.
    McCarron, P.A.
    Keyword
    Anti-Ran-GTPase peptide; Double emulsion; PLGA; Nanoparticle; Breast cancer; Drug delivery
    Rights
    © 2017 Elsevier B.V. Reproduced in accordance with the publisher's self-archiving policy. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
    Peer-Reviewed
    Yes
    
    Metadata
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    Abstract
    Ran is a small ras-related GTPase and is highly expressed in aggressive breast carcinoma. Overexpression induces malignant transformation and drives metastatic growth. We have designed a novel series of anti-Ran-GTPase peptides, which prevents Ran hydrolysis and activation, and although they display effectiveness in silico, peptide activity is suboptimal in vitro due to reduced bioavailability and poor delivery. To overcome this drawback, we delivered an anti-Ran-GTPase peptide using encapsulation in PLGA-based nanoparticles (NP). Formulation variables within a double emulsion solvent evaporation technique were controlled to optimise physicochemical properties. NP were spherical and negatively charged with a mean diameter of 182–277 nm. Peptide integrity and stability were maintained after encapsulation and release kinetics followed a sustained profile. We were interested in the relationship between cellular uptake and poly(ethylene glycol) (PEG) in the NP matrix, with results showing enhanced in vitro uptake with increasing PEG content. Peptide-loaded, pegylated (10% PEG)-PLGA NP induced significant cytotoxic and apoptotic effects in MDA-MB-231 breast cancer cells, with no evidence of similar effects in cells pulsed with free peptide. Western blot analysis showed that encapsulated peptide interfered with the proposed signal transduction pathway of the Ran gene. Our novel blockade peptide prevented Ran activation by blockage of regulator of chromosome condensation 1 (RCC1) following peptide release directly in the cytoplasm once endocytosis of the peptide-loaded nanoparticle has occurred. RCC1 blockage was effective only when a nanoparticulate delivery approach was adopted.
    URI
    http://hdl.handle.net/10454/11435
    Version
    Accepted Manuscript
    Citation
    Haggag YA, Matchett KB, Dakir E-H et al (2017) Nano-encapsulation of a novel anti-Ran- GTPase peptide for blockade of regulator of chromosome condensation 1 (RCC1) function in MDA-MB-231 breast cancer cells. International Journal of Pharmaceutics. 521(1-2): 40-53.
    Link to publisher’s version
    http://dx.doi.org/10.1016/j.ijpharm.2017.02.006
    Type
    Article
    Collections
    Life Sciences Publications

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