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dc.contributor.authorMaguire, John*
dc.contributor.authorParry, Neil R.A.*
dc.contributor.authorKremers, Jan*
dc.contributor.authorMurray, I.J.*
dc.contributor.authorMcKeefry, Declan J.*
dc.date.accessioned2017-02-15T10:06:25Z
dc.date.available2017-02-15T10:06:25Z
dc.date.issued2017-02
dc.identifier.citationMaguire J, Parry NRA, Kremers J et al (2017) The morphology of human rod ERGs obtained by silent substitution stimulation. Documenta Ophthalmologica. 134(1): 11-24.en_US
dc.identifier.urihttp://hdl.handle.net/10454/11360
dc.descriptionYesen_US
dc.description.abstractPurpose To record transient ERGs from the lightadapted human retina using silent substitution stimuli which selectively reflect the activity of rod photoreceptors. We aim to describe the morphology of these waveforms and examine how they are affected by the use of less selective stimuli and by retinal pathology. Methods Rod-isolating stimuli with square-wave temporal profiles (250/250 ms onset/offset) were presented using a 4 primary LED ganzfeld stimulator. Experiment 1: ERGs were recorded using a rodisolating stimulus (63 ph Td, rod contrast, Crod = 0.25) from a group (n = 20) of normal trichromatic observers. Experiment 2: Rod ERGs were recorded from a group (n = 5) using a rodisolating stimulus (Crod = 0.25) which varied in retinal illuminance from 40 to 10,000 ph Td. Experiment 3: ERGs were elicited using 2 kinds of nonisolating stimuli; (1) broadband and (2) rod-isolating stimuli which contained varying degrees of L- and M-cone excitation. Experiment 4: Rod ERGs were recorded from two patient groups with rod monochromacy (n = 3) and CSNB (type 1; n = 2). Results The rod-isolated ERGs elicited from normal subjects had a waveform with a positive onset component followed by a negative offset. Response amplitude was maximal at retinal illuminances\100 ph Td and was virtually abolished at 400 ph Td. The use of non-selective stimuli altered the ERG waveform eliciting more photopic-like ERG responses. Rod ERGs recorded from rod monochromats had similar features to those recorded from normal trichromats, in contrast to those recorded from participants with CSNB which had an electronegative appearance. Conclusions Our results demonstrate that ERGs elicited by silent substitution stimuli can selectively reflect the operation of rod photoreceptors in the normal, light-adapted human retina.en_US
dc.description.sponsorshipDeutsche Forschungsgemeinschaft (DFG) (KR1317/13-1) and Bundesministerium für Bildung und Forschung (BMBF) (01DN14009) provided financial support for JK.en_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttp://dx.doi.org/ 10.1007/s10633-017-9571-4en_US
dc.rightsThe Author(s) 2017. This article is published with open access at Springerlink.com This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http:// creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.en_US
dc.subjectElectroretinograms; Rod photoreceptors; Silent substitutionen_US
dc.titleThe morphology of human rod ERGs obtained by silent substitution stimulationen_US
dc.status.refereedYesen_US
dc.date.Accepted2017-01-05
dc.date.application2017-01-13
dc.typeArticleen_US
dc.type.versionpublished version paperen_US
refterms.dateFOA2018-07-27T01:13:55Z


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