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dc.contributor.authorDeshpande, P.*
dc.contributor.authorOrtega, Í.*
dc.contributor.authorSefat, Farshid*
dc.contributor.authorSangwan, V.S.*
dc.contributor.authorGreen, N.H.*
dc.contributor.authorClaeyssens, F.*
dc.contributor.authorMacNeil, S.*
dc.date.accessioned2017-01-20T15:40:23Z
dc.date.available2017-01-20T15:40:23Z
dc.date.issued2015
dc.identifier.citationDeshpande P, Ortega I, Sefat F, Sangwan V S, Green N, Claeyssens F and MacNeil S (2015) Rocking Media Over Ex Vivo Corneas Improves This Model and Allows the Study of the Effect of Proinflammatory Cytokines on Wound Healing. Investigative Ophthalmology & Visual Science. 56(3): 1553-61.en_US
dc.identifier.urihttp://hdl.handle.net/10454/11157
dc.descriptionyesen_US
dc.description.abstractPurpose.: The aim of this work was to develop an in vitro cornea model to study the effect of proinflammatory cytokines on wound healing. Methods.: Initial studies investigated how to maintain the ex vivo models for up to 4 weeks without loss of epithelium. To study the effect of cytokines, corneas were cultured with the interleukins IL-17A, IL-22, or a combination of IL-17A and IL-22, or lipopolysaccharide (LPS). The effect of IL-17A on wound healing was then examined. Results.: With static culture conditions, organ cultures deteriorated within 2 weeks. With gentle rocking of media over the corneas and carbon dioxide perfusion, the ex vivo models survived for up to 4 weeks without loss of epithelium. The cytokine that caused the most damage to the cornea was IL-17A. Under static conditions, wound healing of the central corneal epithelium occurred within 9 days, but only a single-layered epithelium formed whether the cornea was exposed to IL-17A or not. With rocking of media gently over the corneas, a multilayered epithelium was achieved 9 days after wounding. In the presence of IL-17A, however, there was no wound healing evident. Characterization of the cells showed that wherever epithelium was present, both differentiated cells and highly proliferative cells were present. Conclusions.: We propose that introducing rocking to extend the effective working life of this model and the introduction of IL-17A to this model to induce aspects of inflammation extend its usefulness to study the effects of agents that influence corneal regeneration under normal and inflamed conditions.en_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttp://dx.doi.org/10.1167/iovs.14-15308en_US
dc.rights© 2015 The Authors. Published by ARVO. This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. (http://creativecommons.org/licenses/by-nc-nd/4.0/).en_US
dc.subjectCornea organ culture; Inflammation; Wound healing; Proinflammatory cytokinesen_US
dc.titleRocking Media Over Ex Vivo Corneas Improves This Model and Allows the Study of the Effect of Proinflammatory Cytokines on Wound Healingen_US
dc.status.refereedyesen_US
dc.typeArticleen_US
dc.type.versionPublished versionen_US
refterms.dateFOA2018-07-27T01:47:03Z


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