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dc.contributor.authorSefat, Farshid*
dc.contributor.authorOrtega, Í.*
dc.contributor.authorMcKean, R.*
dc.contributor.authorDeshpande, P.*
dc.contributor.authorRamachandran, C.*
dc.contributor.authorHill, C.J.*
dc.contributor.authorTzokov, S.B.*
dc.contributor.authorClaeyssens, F.*
dc.contributor.authorSangwan, V.S.*
dc.contributor.authorRyan, A.J.*
dc.contributor.authorMacNeil, S.*
dc.date.accessioned2017-01-20T15:35:00Z
dc.date.available2017-01-20T15:35:00Z
dc.date.issued2014
dc.identifier.citationSefat F, Ortega I, McKean R, Deshpande P, Ramachandran C, Hill CJ, Tzokov SB, Claeyssens F, Sangwan VS, Ryan AJ and MacNeil S (2014) An “off-the shelf” Synthetic Membrane to Simplify Regeneration of Damaged Corneas. 2nd Middle East Conference on Biomedical Engineering (MECBME), Doha, Qata. IEEE Conference Publications. DOI: http://dx.doi.org/10.1109/MECBME.2014.6783206en_US
dc.identifier.urihttp://hdl.handle.net/10454/11155
dc.descriptionyesen_US
dc.description.abstractOur overall aim is to develop a synthetic off-the-shelf alternative to human amniotic membrane which is currently used for delivering cultured limbal stem cells to the cornea in patients who suffer scarring of the cornea because of the loss of limbal stem cells. We have recently reported that both cultured cells and limbal explants grow well on electrospun Poly(D,L-lactide-co-glycolide) (PLGA) (44 kg/mol) with a 50:50 ratio of lactide and glycolide and sterilized with γ-irradiation. Prior to undertaking a clinical study our immediate aim now is to achieve long term storage of the membranes in convenient to use packaging. Membranes were electrospun from Poly(D,L-lactide-co-glycolide) (44 kg/mol) with a 50:50 ratio of lactide and glycolide and sterilized with γ-irradiation and then stored dry (with desiccant) for several months at -80°C and -20°C , Room temperature (UK and India), 37°C and 50°C. We explored the contribution of vacuum sealing and the use of a medical grade bag (PET/Foil/LDPE) to achieve a longer shelf life. Confirmation of membranes being suitable for clinical use was obtained by culturing tissue explants on membranes post storage. When scaffolds were stored dry the rate of breakdown was both temperature and time dependent. At -20°C and -80°C there was no change in fiber diameter over 18 months of storage, and membranes were stable for 12 months at 4°C while at 50°C (above the transition temperature for PLGA) scaffolds lost integrity after several weeks. The use of vacuum packaging and a medical grade bag both improved the storage shelf-life of the scaffolds. The impact of temperature on storage is summarized beneath. We report that this synthetic membrane can be used as an off-the-shelf or-out-of-the freezer alternative to the amniotic membrane for corneal regeneration.en_US
dc.language.isoenen_US
dc.rights© 2014 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other worksen_US
dc.subjectSynthetic membrane; Regeneration; Corneas; Poly(D,L-lactide-co-glycolide) (PLGA)en_US
dc.titleAn “off-the shelf” Synthetic Membrane to Simplify Regeneration of Damaged Corneasen_US
dc.status.refereedyesen_US
dc.typeConference paperen_US
dc.type.versionAccepted Manuscripten_US
dc.identifier.doihttps://doi.org/10.1109/MECBME.2014.6783206
refterms.dateFOA2018-07-26T09:42:00Z


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