BRADFORD SCHOLARS

    • Sign in
    View Item 
    •   Bradford Scholars
    • Engineering and Informatics
    • Engineering and Informatics Publications
    • View Item
    •   Bradford Scholars
    • Engineering and Informatics
    • Engineering and Informatics Publications
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of Bradford ScholarsCommunitiesAuthorsTitlesSubjectsPublication DateThis CollectionAuthorsTitlesSubjectsPublication Date

    My Account

    Sign in

    HELP

    Bradford Scholars FAQsCopyright Fact SheetPolicies Fact SheetDeposit Terms and ConditionsDigital Preservation Policy

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    An “off-the shelf” Synthetic Membrane to Simplify Regeneration of Damaged Corneas

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    View/Open
    Main article (649.9Kb)
    Download
    Publication date
    2014
    Author
    Sefat, Farshid
    Ortega, Í.
    McKean, R.
    Deshpande, P.
    Ramachandran, C.
    Hill, C.J.
    Tzokov, S.B.
    Claeyssens, F.
    Sangwan, V.S.
    Ryan, A.J.
    MacNeil, S.
    Show allShow less
    Keyword
    Synthetic membrane; Regeneration; Corneas; Poly(D,L-lactide-co-glycolide) (PLGA)
    Rights
    © 2014 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works
    Peer-Reviewed
    yes
    
    Metadata
    Show full item record
    Abstract
    Our overall aim is to develop a synthetic off-the-shelf alternative to human amniotic membrane which is currently used for delivering cultured limbal stem cells to the cornea in patients who suffer scarring of the cornea because of the loss of limbal stem cells. We have recently reported that both cultured cells and limbal explants grow well on electrospun Poly(D,L-lactide-co-glycolide) (PLGA) (44 kg/mol) with a 50:50 ratio of lactide and glycolide and sterilized with γ-irradiation. Prior to undertaking a clinical study our immediate aim now is to achieve long term storage of the membranes in convenient to use packaging. Membranes were electrospun from Poly(D,L-lactide-co-glycolide) (44 kg/mol) with a 50:50 ratio of lactide and glycolide and sterilized with γ-irradiation and then stored dry (with desiccant) for several months at -80°C and -20°C , Room temperature (UK and India), 37°C and 50°C. We explored the contribution of vacuum sealing and the use of a medical grade bag (PET/Foil/LDPE) to achieve a longer shelf life. Confirmation of membranes being suitable for clinical use was obtained by culturing tissue explants on membranes post storage. When scaffolds were stored dry the rate of breakdown was both temperature and time dependent. At -20°C and -80°C there was no change in fiber diameter over 18 months of storage, and membranes were stable for 12 months at 4°C while at 50°C (above the transition temperature for PLGA) scaffolds lost integrity after several weeks. The use of vacuum packaging and a medical grade bag both improved the storage shelf-life of the scaffolds. The impact of temperature on storage is summarized beneath. We report that this synthetic membrane can be used as an off-the-shelf or-out-of-the freezer alternative to the amniotic membrane for corneal regeneration.
    URI
    http://hdl.handle.net/10454/11155
    Version
    Accepted Manuscript
    Citation
    Sefat F, Ortega I, McKean R, Deshpande P, Ramachandran C, Hill CJ, Tzokov SB, Claeyssens F, Sangwan VS, Ryan AJ and MacNeil S (2014) An “off-the shelf” Synthetic Membrane to Simplify Regeneration of Damaged Corneas. 2nd Middle East Conference on Biomedical Engineering (MECBME), Doha, Qata. IEEE Conference Publications. DOI: http://dx.doi.org/10.1109/MECBME.2014.6783206
    Link to publisher’s version
    http://dx.doi.org/10.1109/MECBME.2014.6783206
    Type
    Conference paper
    Collections
    Engineering and Informatics Publications

    entitlement

     
    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.