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dc.contributor.authorMahjour, S.B.*
dc.contributor.authorFu, X.*
dc.contributor.authorYang, X.*
dc.contributor.authorFong, J.*
dc.contributor.authorSefat, Farshid*
dc.contributor.authorWang, H.*
dc.date.accessioned2017-01-20T15:27:57Z
dc.date.available2017-01-20T15:27:57Z
dc.date.issued2015
dc.identifier.citationMahjour SB, Fu X, Yang X, Fong J, Sefat F, Wang H (2015) Rapid creation of skin substitutes from human skin cells and biomimetic nanofibers for acute full-thickness wound repair. Burns. 41 (8): 1764–1774.
dc.identifier.urihttp://hdl.handle.net/10454/11151
dc.descriptionYes
dc.description.abstractCreation of functional skin substitutes within a clinically acceptable time window is essential for timely repair and management of large wounds such as extensive burns. The aim of this study was to investigate the possibility of fabricating skin substitutes via a bottom-up nanofiber-enabled cell assembly approach and using such substitutes for full-thickness wound repair in nude mice. Following a layer-by-layer (L-b-L) manner, human primary skin cells (fibroblasts and keratinocytes) were rapidly assembled together with electrospun polycaprolactone (PCL)/collagen (3:1 w/w, 8% w/v) nanofibers into 3D constructs, in which fibroblasts and keratinocytes were located in the bottom and upper portion respectively. Following culture, the constructs developed into a skin-like structure with expression of basal keratinocyte markers and deposition of new matrix while exhibited good mechanical strength (as high as 4.0 MPa by 14 days). Treatment of the full-thickness wounds created on the back of nude mice with various grafts (acellular nanofiber meshes, dermal substitutes, skin substitutes and autografts) revealed that 14-day-cultured skin substitutes facilitated a rapid wound closure with complete epithelialization comparable to autografts. Taken together, skin-like substitutes can be formed by L-b-L assembling human skin cells and biomimetic nanofibers and they are effective to heal acute full-thickness wounds in nude mice.
dc.language.isoenen
dc.rights© 2015 Elsevier. Reproduced in accordance with the publisher's self-archiving policy. This manuscript version is made available under the CC-BY-NC-ND 4.0 license.
dc.subjectSkin tissue engineering
dc.subjectLayer-by-layer
dc.subjectWound healing
dc.subjectPCL/collagen polyblend nanofibers
dc.subjectHuman skin cells
dc.subjectBiomimetic nanofibers
dc.titleRapid creation of skin substitutes from human skin cells and biomimetic nanofibers for acute full-thickness wound repair
dc.status.refereedYes
dc.typeArticle
dc.type.versionAccepted manuscript
dc.identifier.doihttps://doi.org/10.1016/j.burns.2015.06.011
dc.rights.licenseCC-BY-NC-ND
refterms.dateFOA2018-07-26T09:41:22Z
dc.openaccess.statusopenAccess


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