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dc.contributor.authorMancini, S.J.
dc.contributor.authorWhite, A.D.
dc.contributor.authorBijland, S.
dc.contributor.authorRutherford, C.
dc.contributor.authorGraham, D.
dc.contributor.authorRichter, E.A.
dc.contributor.authorViollet, B.
dc.contributor.authorTouyz, R.M.
dc.contributor.authorPalmer, Timothy M.
dc.contributor.authorSalt, I.P.
dc.date.accessioned2016-12-07T16:05:22Z
dc.date.available2016-12-07T16:05:22Z
dc.date.issued2017-01-15
dc.identifier.citationMancini SJ, White AD, Bijland S, Rutherford C, Graham D, Richter EA, Viollet B, Touyz RM, Palmer TM, Salt IP (2016) Activation of AMP-activated protein kinase rapidly suppresses multiple pro-inflammatory pathways in adipocytes including IL-1 receptor-associated kinase-4 phosphorylation. Molecular and Cellular Endocrinology. 440: 44-56.en_US
dc.identifier.urihttp://hdl.handle.net/10454/10903
dc.descriptionyesen_US
dc.description.abstractInflammation of adipose tissue in obesity is associated with increased IL-1β, IL-6 and TNF-α secretion and proposed to contribute to insulin resistance. AMP-activated protein kinase (AMPK) regulates nutrient metabolism and is reported to have anti-inflammatory actions in adipose tissue, yet the mechanisms underlying this remain poorly characterised. The effect of AMPK activation on cytokine-stimulated proinflammatory signalling was therefore assessed in cultured adipocytes. AMPK activation inhibited IL-1β-stimulated CXCL10 secretion, associated with reduced interleukin-1 receptor associated kinase-4 (IRAK4) phosphorylation and downregulated MKK4/JNK and IKK/IκB/NFκB signalling. AMPK activation inhibited TNF-α-stimulated IKK/IκB/NFκB signalling but had no effect on JNK phosphorylation. The JAK/STAT3 pathway was also suppressed by AMPK after IL-6 stimulation and during adipogenesis. Adipose tissue from AMPKα1−/− mice exhibited increased JNK and STAT3 phosphorylation, supporting suppression of these distinct proinflammatory pathways by AMPK in vivo. The inhibition of multiple pro-inflammatory signalling pathways by AMPK may underlie the reported beneficial effects of AMPK activation in adipose tissue.en_US
dc.description.sponsorshipBritish Heart Foundationen_US
dc.language.isoenen_US
dc.relation.isreferencedbyhttp://dx.doi.org/10.1016/j.mce.2016.11.010en_US
dc.rights© 2016 The Authors. Published by Elsevier Ireland Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).en_US
dc.subjectAdipocyte; AMP-activated protein kinase; Inflammation; Signallingen_US
dc.titleActivation of AMP-activated protein kinase rapidly suppresses multiple pro-inflammatory pathways in adipocytes including IL-1 receptor-associated kinase-4 phosphorylationen_US
dc.status.refereedyesen_US
dc.date.Accepted2016-11-10
dc.date.application2016-11-11
dc.typeArticleen_US
dc.type.versionPublished versionen_US
refterms.dateFOA2018-07-26T09:21:26Z


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