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dc.contributor.authorAl-Jammal, M.K.H.*
dc.contributor.authorAl Ayoub, Yuosef*
dc.contributor.authorAssi, Khaled H.*
dc.date.accessioned2016-11-28T15:06:12Z
dc.date.available2016-11-28T15:06:12Z
dc.date.issued2015-03-03
dc.identifier.citationAl-Jammal MKH, Al Ayoub Y, Assi KH (2015) Development and Validation of Micro Emulsion High Performance Liquid Chromatography(MELC) Method for the Determination of Nifedipine in Pharmaceutical Preparation. Pharmeutica Analytica Acta. 6(3)en_US
dc.identifier.urihttp://hdl.handle.net/10454/10709
dc.descriptionYesen_US
dc.description.abstractMicroemulsion is a stable, isotropic clear solution consisting of oil based substance, water surfactant and cosurfactant. There are two types of microemulsion which are used as a mobile phase; water in oil (w/o) and oil in water (o/w).Microemulsion has a strong ability to solubilize both hydrophobic and hydrophilic analytes, therefore reducing the pre-treatment of the sample which is needed for the complex sample. Recent reports found that separating the analytes by using microemulsion high performance liquid chromatography can be achieved with superior speed and efficiency compared to conventional HPLC modes. In this work, Oil in water (o/w) microemulsion has been used for the determination of nifedipine in pharmaceutical preparation. The effect of each parameter on the separation process was examined. The samples were injected into C18, analytical columns maintained at 30°C with a flow rate 1 ml/min. The mobile phase was 87.1% aqueous orthophosphate buffer 15 mM (adjusted to pH 3 with orthophosphoric acid), 0.8% of octane as oil, 4.5 SDS, and 7.6% 1-butanol, all w/w. The nifedipine and internal standard peaks were detected by UV detection at λ max 237 nm The calibration curve was linear (r2=0.9995) over nifedipine concentrations ranging from 1 to 60 μg/ml (n=6). The method has good sensitivity with limit of detection (LOD) of 0.33 μg/ml and limit of quantitation (LOQ) of 1.005 μg/ ml. Also it has an excellent accuracy ranging from 99.11 to 101.64%. The intra-day and inter-day precisions (RSD %) were <0.45% and <0.9%, respectively.en_US
dc.language.isoenen_US
dc.rights© 2015 Al-Jammal MKH, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.subjectHigh-performance liquid chromatography; Microemulsion; Determination; Validation; Nifedipineen_US
dc.titleDevelopment and Validation of Micro Emulsion High Performance Liquid Chromatography(MELC) Method for the Determination of Nifedipine in Pharmaceutical Preparationen_US
dc.status.refereedYesen_US
dc.date.Accepted2015-02-24
dc.typeArticleen_US
dc.type.versionPublished versionen_US
dc.identifier.doihttps://doi.org/10.4172/2153-2435.1000347
refterms.dateFOA2018-07-26T09:16:33Z


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