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dc.contributor.authorYoussef, M.M.*
dc.contributor.authorAl-Omair, M.A.*
dc.contributor.authorPicksley, Stephen M.*
dc.date.accessioned2016-11-23T17:56:33Z
dc.date.available2016-11-23T17:56:33Z
dc.date.issued2014
dc.identifier.citationYoussef MM, Al-Omair MA and Picksley SM (2014) Genetic characterisation of Escherichia coli RecN protein as a member of SMC family of proteins. Arabian Journal of Chemistry. 7(3): 327-334.
dc.identifier.urihttp://hdl.handle.net/10454/10558
dc.descriptionYes
dc.description.abstractThe proteins of SMC family are characterised by having Walker A and B sites. The Escherichia coli RecN protein is a prokaryotic member of SMC family that involved in the induced excision of Tn10 and the repair of the DNA double strand breaks. In this work, the Walker A nucleotide binding site of the E. coli RecN protein was mutated by changing the highly conserved lysine residue 35 to the aspartic acid (D), designated as recN(K35D). Reverse genetics was utilized to delete the entire recN gene (Delta recN108) or introduce the recN(K35D) gene into the E. coli chromosomal DNA. The recN(K35D) cells showed decreasing in the frequency of excision of Tn10 from gal76
dc.relation.isreferencedbyhttps://doi.org/10.1016/j.arabjc.2011.02.001
dc.rights(c) 2014 Elsevier. This is an Open Access article distributed under the Creative Commons BY-NC-ND license (https://creativecommons.org/licenses/by-nc-nd/3.0/)
dc.subjectRECN; SMC; tn10 excision; Recombination; Recombination-deficient mutants; Double-strand breaks; DNA-repair; Chromosome condensation; Exonuclease-i; k-12; Identification; Mutations; Strains; Product
dc.titleGenetic characterisation of Escherichia coli RecN protein as a member of SMC family of proteins
dc.status.refereedYes
dc.date.Accepted2011-02-01
dc.date.application2011-02-6
dc.typeArticle
dc.type.versionPublished version
refterms.dateFOA2018-07-26T09:14:30Z


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