Genetic characterisation of Escherichia coli RecN protein as a member of SMC family of proteins

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Publication date
2014
Author
Youssef, M.M.
Al-Omair, M.A.
Picksley, Stephen M.
Keyword
RECN; SMC; tn10 excision; Recombination; Recombination-deficient mutants; Double-strand breaks; DNA-repair; Chromosome condensation; Exonuclease-i; k-12; Identification; Mutations; Strains; Product
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(c) 2014 Elsevier. This is an Open Access article distributed under the Creative Commons BY-NC-ND license (https://creativecommons.org/licenses/by-nc-nd/3.0/)
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Abstract
The proteins of SMC family are characterised by having Walker A and B sites. The Escherichia coli RecN protein is a prokaryotic member of SMC family that involved in the induced excision of Tn10 and the repair of the DNA double strand breaks. In this work, the Walker A nucleotide binding site of the E. coli RecN protein was mutated by changing the highly conserved lysine residue 35 to the aspartic acid (D), designated as recN(K35D). Reverse genetics was utilized to delete the entire recN gene (Delta recN108) or introduce the recN(K35D) gene into the E. coli chromosomal DNA. The recN(K35D) cells showed decreasing in the frequency of excision of Tn10 from gal76
URI
http://hdl.handle.net/10454/10558
Version
Published version
Citation
Youssef MM, Al-Omair MA and Picksley SM (2014) Genetic characterisation of Escherichia coli RecN protein as a member of SMC family of proteins. Arabian Journal of Chemistry. 7(3): 327-334.
Link to publisher’s version
https://doi.org/10.1016/j.arabjc.2011.02.001
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Article
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