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dc.contributor.authorSarker, P.
dc.contributor.authorSwindells, K.
dc.contributor.authorDouglas, C.W.I.
dc.contributor.authorMacNeil, S.
dc.contributor.authorRimmer, Stephen
dc.contributor.authorSwanson, L.
dc.date.accessioned2016-11-23T17:54:34Z
dc.date.available2016-11-23T17:54:34Z
dc.date.issued2014
dc.identifier.citationSarker P, Swindells K, Douglas CWI et al (2014) Förster resonance energy transfer confirms the bacterial-induced conformational transition in highly-branched poly(N-isopropyl acrylamide with vancomycin end groups on binding to Staphylococcus aureus. Soft Matter. 10(31): 5824-5835.
dc.identifier.urihttp://hdl.handle.net/10454/10525
dc.descriptionNo
dc.description.abstractWe describe a series of experiments designed to investigate the conformational transition that highly-branched polymers with ligands undergo when interacting with bacteria, a process that may provide a new sensing mechanism for bacterial detection. Fluorescent highly-branched poly(N-isopropyl acrylamide)s (HB-PNIPAM) were prepared by sequential self-condensing radical copolymerizations, using anthrylmethyl methacrylate (AMMA) and fluorescein-O-acrylate (FA) as fluorescent comonomers and 4-vinylbenzyl pyrrole carbodithioate as a branch forming monomer. Differences in reactivity necessitated to first copolymerize AMMA then react with FA in a separate sequential monomer feed step. Modifications of the chain ends produced vancomycin-functional derivatives (HB-PNIPAM-Van). The AMMA and FA labels allow probing of the conformational behaviour of the polymers in solution via Forster resonance energy transfer experiments. It was shown that interaction of this polymer's end groups with Staphylococcus aureus induced a macromolecular collapse. The data thus provide conclusive evidence for a conformational transition that is driven by binding to a bacterium.
dc.relation.isreferencedbyhttps://doi.org/10.1039/c4sm00056k
dc.subjectAcrylic Resins; Fluorescein; Fluorescence resonance energy transfer; Models; Molecular conformation; Molecular structure; Solutions; Staphylococcus aureus; Temperature; Vancomycin
dc.titleFörster resonance energy transfer confirms the bacterial-induced conformational transition in highly-branched poly(N-isopropyl acrylamide with vancomycin end groups on binding to Staphylococcus aureus
dc.status.refereedYes
dc.date.Accepted2014-06-13
dc.typeArticle
dc.type.versionNo full-text available in the repository


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