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    Förster resonance energy transfer confirms the bacterial-induced conformational transition in highly-branched poly(N-isopropyl acrylamide with vancomycin end groups on binding to Staphylococcus aureus

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    Publication date
    2014
    Author
    Sarker, P.
    Swindells, K.
    Douglas, C.W.I.
    MacNeil, S.
    Rimmer, Stephen
    Swanson, L.
    Keyword
    Acrylic Resins; Fluorescein; Fluorescence resonance energy transfer; Models; Molecular conformation; Molecular structure; Solutions; Staphylococcus aureus; Temperature; Vancomycin
    Peer-Reviewed
    Yes
    
    Metadata
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    Abstract
    We describe a series of experiments designed to investigate the conformational transition that highly-branched polymers with ligands undergo when interacting with bacteria, a process that may provide a new sensing mechanism for bacterial detection. Fluorescent highly-branched poly(N-isopropyl acrylamide)s (HB-PNIPAM) were prepared by sequential self-condensing radical copolymerizations, using anthrylmethyl methacrylate (AMMA) and fluorescein-O-acrylate (FA) as fluorescent comonomers and 4-vinylbenzyl pyrrole carbodithioate as a branch forming monomer. Differences in reactivity necessitated to first copolymerize AMMA then react with FA in a separate sequential monomer feed step. Modifications of the chain ends produced vancomycin-functional derivatives (HB-PNIPAM-Van). The AMMA and FA labels allow probing of the conformational behaviour of the polymers in solution via Forster resonance energy transfer experiments. It was shown that interaction of this polymer's end groups with Staphylococcus aureus induced a macromolecular collapse. The data thus provide conclusive evidence for a conformational transition that is driven by binding to a bacterium.
    URI
    http://hdl.handle.net/10454/10525
    Version
    No full-text available in the repository
    Citation
    Sarker P, Swindells K, Douglas CWI et al (2014) Förster resonance energy transfer confirms the bacterial-induced conformational transition in highly-branched poly(N-isopropyl acrylamide with vancomycin end groups on binding to Staphylococcus aureus. Soft Matter. 10(31): 5824-5835.
    Link to publisher’s version
    https://doi.org/10.1039/c4sm00056k
    Type
    Article
    Collections
    Life Sciences Publications

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