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    Potential and limits of Raman spectroscopy for carotenoid detection in microorganisms: implications for astrobiology

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    Publication date
    2014-12-13
    Author
    Jehlička, J.
    Edwards, Howell G.M.
    Osterrothova, K.
    Novotna, J.
    Nedbalova, L.
    Kopecky, J.
    Nemec, I.
    Oren, A.
    Keyword
    Raman spectroscopy; Algae; Bacteria; Cyanobacteria; Detecting carotenoids; High-performance liquid chromatography
    Peer-Reviewed
    Yes
    
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    Abstract
    In this paper, it is demonstrated how Raman spectroscopy can be used to detect different carotenoids as possible biomarkers in various groups of microorganisms. The question which arose from previous studies concerns the level of unambiguity of discriminating carotenoids using common Raman microspectrometers. A series of laboratory-grown microorganisms of different taxonomic affiliation was investigated, such as halophilic heterotrophic bacteria, cyanobacteria, the anoxygenic phototrophs, the non-halophilic heterotrophs as well as eukaryotes (Ochrophyta, Rhodophyta and Chlorophyta). The data presented show that Raman spectroscopy is a suitable tool to assess the presence of carotenoids of these organisms in cultures. Comparison is made with the high-performance liquid chromatography approach of analysing pigments in extracts. Direct measurements on cultures provide fast and reliable identification of the pigments. Some of the carotenoids studied are proposed as tracers for halophiles, in contrast with others which can be considered as biomarkers of other genera. The limits of application of Raman spectroscopy are discussed for a few cases where the current Raman spectroscopic approach does not allow discriminating structurally very similar carotenoids. The database reported can be used for applications in geobiology and exobiology for the detection of pigment signals in natural settings.
    URI
    http://hdl.handle.net/10454/10466
    Version
    No full-text available in the repository
    Citation
    Jehlička J, Edwards HGM, Osterrothova K et al (2014) Potential and limits of Raman spectroscopy for carotenoid detection in microorganisms: implications for astrobiology. Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences. 372(2030): 20140199.
    Link to publisher’s version
    https://doi.org/10.1098/rsta.2014.0199
    Type
    Article
    Collections
    Life Sciences Publications

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