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dc.contributor.authorAli, Aftab H.M.*
dc.contributor.authorKurzawa-Zegota, Malgorzata*
dc.contributor.authorNajafzadeh, Mojgan*
dc.contributor.authorGopalan, Rajendran C.*
dc.contributor.authorPlewa, M.J.*
dc.contributor.authorAnderson, Diana*
dc.date.accessioned2016-11-21T15:53:45Z
dc.date.available2016-11-21T15:53:45Z
dc.date.issued2014-12
dc.identifier.citationAli A, Kurzawa-Zegota M, Najafzadeh M et al (2014) Effect of drinking water disinfection by-products in human peripheral blood lymphocytes and sperm. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 770: 136-143.
dc.identifier.urihttp://hdl.handle.net/10454/10412
dc.descriptionNo
dc.description.abstractDrinking water disinfection by-products (DBPs) are generated by the chemical disinfection of water and may pose hazards to public health. Two major classes of DBPs are found in finished drinking water: haloacetic acids (HAAs) and trihalomethanes (THMs). HAAs are formed following disinfection with chlorine, which reacts with iodide and bromide in the water. Previously the HAAs were shown to be cytotoxic, genotoxic, mutagenic, teratogenic and carcinogenic. OBJECTIVES: To determine the effect of HAAs in human somatic and germ cells and whether oxidative stress is involved in genotoxic action. In the present study both somatic and germ cells have been examined as peripheral blood lymphocytes and sperm. The effects of three HAA compounds: iodoacetic acid (IAA), bromoacetic acid (BAA) and chloroacetic acid (CAA) were investigated. After determining appropriate concentration responses, oxygen radical involvement with the antioxidants, butylated hydroxanisole (BHA) and the enzyme catalase, were investigated in the single cell gel electrophoresis (Comet) assay under alkaline conditions, >pH 13 and the micronucleus assay. In the Comet assay, BHA and catalase were able to reduce DNA damage in each cell type compared to HAA alone. In the micronucleus assay, micronuclei (MNi) were found in peripheral lymphocytes exposed to all three HAAs and catalase and BHA were in general, able to reduce MNi induction, suggesting oxygen radicals play a role in both assays. These observations are of concern to public health since both human somatic and germ cells show similar genotoxic responses.
dc.language.isoen
dc.subjectBromoacetic acid
dc.subjectBAA
dc.subjectButylated hydroxyanisole
dc.subjectBHA
dc.subjectCBMN (Cytokinesis-block micronucleus) assay
dc.subjectCatalase
dc.subjectChloroacetic acid
dc.subjectCAA
dc.subjectComet assay
dc.subjectHaloacetic acid
dc.subjectHAA
dc.subjectIodoacetic acid
dc.subjectIAA
dc.subjectMicronuclei
dc.subjectMNi
dc.titleEffect of drinking water disinfection by-products in human peripheral blood lymphocytes and sperm
dc.status.refereedYes
dc.date.Accepted2014-08-20
dc.date.application2014-08-26
dc.typeArticle
dc.type.versionNo full-text in the repository
dc.identifier.doihttps://doi.org/10.1016/j.mrfmmm.2014.08.003
dc.openaccess.statusclosedAccess


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