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dc.contributor.authorDrake, Philip*
dc.contributor.authorChen, Y-C.*
dc.contributor.authorLehmann, I.*
dc.contributor.authorJiang, P-S.*
dc.date.accessioned2016-11-15T11:27:00Z
dc.date.available2016-11-15T11:27:00Z
dc.date.issued2015-08
dc.identifier.citationDrake P, Chen Y-C, Lehmann I et al (2015) Nanoparticle labels for pathogen detection through nucleic acid amplification tests. Microfluidics and Nanofluidics. 19(2): 299-305.
dc.identifier.urihttp://hdl.handle.net/10454/10333
dc.descriptionYes
dc.description.abstractMagnetic nanoparticles and surface-enhanced Raman scattering (SERS) active nanoparticles were coated with short chain DNA tags. These were then used to identify a target bacterial DNA sequence. The tags function as primers in a standard PCR with the reverse primers and forward primers on the SERS nanoparticles and magnetic nanoparticles, respectively. During the PCR cycles, a composite nanostructure is formed that is both magnetically responsive and SERS active. After magnetic trapping, the intensity of the SERS signal can be related back to the concentration of the target DNA. A test assay was performed that showed a detection limit (based on the signal to noise ratio) of less than 3 zeptomole (41 pg/L). For comparison, a PCR assay based on the standard SYBR Green method was performed. This used the same primers and target DNA and had a detection limit of 10 attomoles (138 ng/L), 3,000 times less sensitive. The work documents the proof of principle study and shows for the first time the use of SERS-NP labels in the quantification of nucleic acid amplification tests and PCR.
dc.language.isoen
dc.rightsThe final publication is available at Springer via http://dx.doi.org/10.1007/s10404-014-1530-8
dc.subjectNanoparticle
dc.subjectRaman
dc.subjectMagnetic
dc.subjectDNA
dc.subjectPrimer
dc.subjectPCR
dc.subjectNucleic acid
dc.titleNanoparticle labels for pathogen detection through nucleic acid amplification tests
dc.status.refereedYes
dc.date.Accepted2014-12-02
dc.date.application2014-12-20
dc.typeArticle
dc.type.versionAccepted manuscript
dc.identifier.doihttps://doi.org/10.1007/s10404-014-1530-8
dc.rights.licenseUnspecified
refterms.dateFOA2018-07-26T09:09:43Z
dc.openaccess.statusopenAccess


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