Characterisation of SEQ0694 (PrsA/PrtM) of Streptococcus equi as a functional peptidyl-prolyl isomerase affecting multiple secreted protein substrates

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2015Rights
© 2015 Royal Society of Chemistry. This is an Open Access article published under the Creative Commons CC-BY license (http://creativecommons.org/licenses/by/3.0/)Peer-Reviewed
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openAccessAccepted for publication
08/10/2015
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Peptidyl-prolyl isomerase (PPIase) lipoproteins have been shown to influence the virulence of a number of Gram-positive bacterial human and animal pathogens, most likely through facilitating the folding of cell envelope and secreted virulence factors. Here, we used a proteomic approach to demonstrate that the Streptococcus equi PPIase SEQ0694 alters the production of multiple secreted proteins, including at least two putative virulence factors (FNE and IdeE2). We demonstrate also that, despite some unusual sequence features, recombinant SEQ0694 and its central parvulin domain are functional PPIases. These data add to our knowledge of the mechanisms by which lipoprotein PPIases contribute to the virulence of streptococcal pathogens.Version
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Ikolo F, Zhang M, Harrison DJ et al (2015) Characterisation of SEQ0694 (PrsA/PrtM) of Streptococcus equi as a functional peptidyl-prolyl isomerase affecting multiple secreted protein substrates. Molecular BioSystems. 11(12): 3279-3286.Link to Version of Record
https://doi.org/10.1039/c5mb00543dType
Articleae974a485f413a2113503eed53cd6c53
https://doi.org/10.1039/c5mb00543d