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    The comet assay in human biomonitoring

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    Publication date
    2013
    Author
    Anderson, Diana
    Dhawan, A.
    Laubenthal, Julian
    Keyword
    Comet assay; Human biomonitoring
    Peer-Reviewed
    Yes
    
    Metadata
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    Abstract
    Human biomonitoring studies aim to identify potential exposures to environmental, occupational, or lifestyle toxicants in human populations and are commonly used by public health decision makers to predict disease risk. The Comet assay measures changes in genomic stability and is one of the most reliable biomarkers to indicate early biological effects, and therefore accepted by various governmental regulatory agencies. The appeal of the Comet assay lies in its relative simplicity, rapidity, sensitivity, and economic efficiency. Furthermore, the assay is known for its broad versatility, as it can be applied to virtually any human cell and easily adapted in order to detect particular biomarkers of interest, such as DNA repair capacity or single- and double-strand breaks. In a standard experiment, isolated single cells are first embedded in agarose, and then lysed in high-salt solutions in order to remove all cellular contents except the DNA attached to a nuclear scaffold. Subsequent electrophoresis results in accumulation of undamaged DNA sequences at the proximity of the nuclear scaffold, while damaged sequences migrate towards the anode. When visualized with fluorochromes, these migrated DNA fragments resemble a comet tail and can be quantified for their intensity and shape according to internationally drafted guidelines.
    URI
    http://hdl.handle.net/10454/10114
    Version
    No full-text available in the repository
    Citation
    Anderson D, Dhawan A and Laubenthal J (2013) The comet assay in human biomonitoring. In: Dhawan A and Bajpayee M (Eds) Genotoxicity Assessment. (Methods in Molecular Biology) Totowa, NJ, USA: Humana Press: 347-362.
    Link to publisher’s version
    http://dx.doi.org/10.1007/978-1-62703-529-3_18
    Type
    Book chapter
    Collections
    Life Sciences Publications

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