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dc.contributor.authorRiches-Suman, Kirsten*
dc.contributor.authorWarburton, P.*
dc.contributor.authorO'Regan, D.J.*
dc.contributor.authorTurner, N.A.*
dc.contributor.authorPorter, K.E.*
dc.date.accessioned2016-10-26T15:50:54Z
dc.date.available2016-10-26T15:50:54Z
dc.date.issued2014-04
dc.identifier.citationRiches-Suman K, Warburton P, O’Regan DJ et al (2014) Type 2 diabetes impairs venous, but not arterial smooth muscle cell function: possible role of differential RhoA activity. Cardiovascular Revascularization Medicine. 15(3):141-148.
dc.identifier.urihttp://hdl.handle.net/10454/10086
dc.descriptionYes
dc.description.abstractBackground/purpose Coronary heart disease is the leading cause of morbidity in patients with type 2 diabetes mellitus (T2DM), frequently resulting in a requirement for coronary revascularization using the internal mammary artery (IMA) or saphenous vein (SV). Patency rates of SV grafts are inferior to IMA and further impaired by T2DM whilst IMA patencies appear similar in both populations. Smooth muscle cells (SMC) play a pivotal role in graft integration; we therefore examined the phenotype and proliferative function of IMA- and SV-SMC isolated from non-diabetic (ND) patients or those diagnosed with T2DM. Methods/materials SMC were cultured from fragments of SV or IMA. Morphology was analyzed under light microscopy (spread cell area measurements) and confocal microscopy (F-actin staining). Proliferation was analyzed by cell counting. Levels of RhoA mRNA, protein and activity were measured by real-time RT-PCR, western blotting and G-LISA respectively. Results IMA-SMC from T2DM and ND patients were indistinguishable in both morphology and function. By comparison, SV-SMC from T2DM patients exhibited significantly larger spread cell areas (1.5-fold increase, P < 0.05), truncated F-actin fibers and reduced proliferation (33% reduction, P < 0.05). Furthermore, lower expression and activity of RhoA were observed in SV-SMC of T2DM patients (37% reduction in expression, P < 0.05 and 43% reduction in activity, P < 0.01). Conclusions IMA-SMC appear impervious to phenotypic modulation by T2DM. In contrast, SV-SMC from T2DM patients exhibit phenotypic and functional changes accompanied by reduced RhoA activity. These aberrancies may be epigenetic in nature, compromising SMC plasticity and SV graft adaptation in T2DM patients.
dc.language.isoen
dc.rights© 2014 Elsevier. Reproduced in accordance with the publisher's self-archiving policy. This manuscript version is made available under the CC-BY-NC-ND 4.0 license.
dc.subjectType 2 diabetes
dc.subjectSmooth muscle cell
dc.subjectSaphenous vein
dc.subjectInternal mammary artery
dc.subjectRhoA
dc.subjectCell phenotype
dc.titleType 2 diabetes impairs venous, but not arterial smooth muscle cell function: possible role of differential RhoA activity
dc.status.refereedYes
dc.date.Accepted2014-02-20
dc.date.application2014-03-02
dc.typeArticle
dc.type.versionAccepted manuscript
dc.identifier.doihttps://doi.org/10.1016/j.carrev.2014.02.005
dc.rights.licenseCC-BY-NC-ND
refterms.dateFOA2018-07-27T02:03:04Z
dc.openaccess.statusopenAccess


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