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Cellular uptake and efflux of palbociclib in vitro in single cell and spheroid models
Jove, M. Spencer, Jade A. Hubbard, M.E. Holden, E.C. O'Dea, R.D. Brook, B.S. Phillips, Roger M. Smye, S.W. Twelves, C.J.
Jove, M.
Spencer, Jade A.
Hubbard, M.E.
Holden, E.C.
O'Dea, R.D.
Brook, B.S.
Phillips, Roger M.
Smye, S.W.
Twelves, C.J.
Publication Date
2019-08-01
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2019-06-06
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Abstract
Adequate drug distribution through tumours is essential for treatment to be effective. Palbociclib is a cyclin-dependent kinase (CDK) 4/6 inhibitor approved for use in patients with hormone receptor (HR) positive, HER2 negative metastatic breast cancer (BC). It has unusual physicochemical properties, which may significantly influence its distribution in tumour tissue. We studied the penetration and distribution of palbociclib in vitro, including the use of multicellular three-dimensional models and mathematical modelling. MCF-7 and DLD-1 cell lines were grown as single cell suspensions (SCS) and spheroids; palbociclib uptake and efflux were studied using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Intracellular concentrations of palbociclib for MCF-7 SCS (Cmax 3.22 µM) and spheroids (Cmax 2.91 µM) were 32 and 29 fold higher and in DLD-1, 13 and 7 fold higher, respectively than the media concentration (0.1 µM). Total palbociclib uptake was lower in DLD-1 cells than MCF-7 cells both in SCS and in spheroids. Both uptake and efflux of palbociclib were slower in spheroids than SCS. These data were used to develop a mathematical model of palbociclib transport that quantifies key parameters determining drug penetration and distribution. The model reproduced qualitatively most features of the experimental data and distinguished between SCS and spheroids, providing additional support for hypotheses derived from the experimental data. Mathematical modelling has the potential for translating in vitro data into clinically relevant estimates of tumour drug concentrations.
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Accepted manuscript
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Jove M, Spencer JA, Hubbard ME et al (2019) Cellular uptake and efflux of palbociclib in vitro in single cell and spheroid models. The Journal of Pharmacology and Experimental Therapeutics. 370(2): 242-251.
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