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Time-resolved crystallography using the Hadamard transform
Yorke, Briony A. Beddard, G.S. Owen, R.L. Pearson, A.R.
Yorke, Briony A.
Beddard, G.S.
Owen, R.L.
Pearson, A.R.
Publication Date
2014-11
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© 2014, Springer Nature. Reproduced in accordance with the publisher's self-archiving policy.
The final publication is available at https://doi.org/10.1038/nmeth.3139.
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openAccess
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04/09/2014
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Abstract
We describe a method for performing time-resolved X-ray crystallographic experiments based on the Hadamard transform, in which time resolution is defined by the underlying periodicity of the probe pulse sequence, and signal/noise is greatly improved over that for the fastest pump-probe experiments depending on a single pulse. This approach should be applicable on standard synchrotron beamlines and will enable high-resolution measurements of protein and small-molecule structural dynamics. It is also applicable to other time-resolved measurements where a probe can be encoded, such as pump-probe spectroscopy.
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Citation
Yorke BA, Beddard GS, Owen RL et al (2014) Time-resolved crystallography using the Hadamard transform. Nature Methods. 11: 1131-1134.
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