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IL-36γ has proinflammatory effects on human endothelial cells

Bridgewood, Charlie
; 
Stacey, M.
; 
Alase, Adewonuola A.
; 
Lagos, D.
; ; 
Wittmann, Miriam
Publication Date
2017-02
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Keywords
Psoriasis, Endothelial cells, Skin, Inflammation, IL-36γ
Rights
© 2017 Wiley This is the peer reviewed version of the following article: Bridgewood C, Stacey M, Alase A, Lagos D, Graham A and Wittmann M (2016) IL-36γ has proinflammatory effects on human endothelial cells. Experimental Dermatology. 26(5): 402-408, which has been published in final form at https://doi.org/10.1111/exd.13228. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
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Yes
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openAccess
Accepted for publication
2016-09-14
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Abstract
Interleukin-36 cytokines are predominantly expressed by epithelial cells. Significant upregulation of epidermal IL-36 is now a recognised characteristic of psoriatic skin inflammation. IL-36 is known to induce inflammatory responses in dendritic cells, fibroblasts and epithelial cells. Although vascular alterations are a hallmark of psoriatic lesions and dermal endothelial cells are well known to play a critical role in skin inflammation, the effects of IL-36 on endothelial cells are unexplored. We here show that endothelial cells including dermal microvascular cells express a functionally active IL-36 receptor. Adhesion molecules VCAM-1 and ICAM-1 are upregulated by IL-36γ stimulation and this is reversed by the presence of the endogenous IL-36 receptor antagonist. IL-36γ stimulated endothelial cells secrete the proinflammatory chemokines IL-8, CCL2 and CCL20. Chemotaxis assays showed increased migration of T cells following IL-36γ stimulation of endothelial cells. These results suggest a role for IL-36γ in the dermal vascular compartment and it is likely to enhance psoriatic skin inflammation by activating endothelial cells and promoting leukocyte recruitment.
URI
http://hdl.handle.net/10454/9483
Version
Accepted manuscript
Citation
Bridgewood C, Stacey M, Alase A, Lagos D, Graham A and Wittmann M (2016) IL-36γ has proinflammatory effects on human endothelial cells. Experimental Dermatology. 26(5): 402-408.
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Link to Version of Record
https://doi.org/10.1111/exd.13228
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Article
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