The mutagenic activity of ethylmethanesulphonate, benzidine and benzo[a]pyrene at the hprt locus of wild-type L5178Y mouse lymphoma cells
Kennelly, J.C. Clare, C.B. Campbell, J. Lane, M.P. Harrington, Dean J. Cole, H. Garner, R.C.
Kennelly, J.C.
Clare, C.B.
Campbell, J.
Lane, M.P.
Harrington, Dean J.
Cole, H.
Garner, R.C.
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1990
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Ethylmethanesulphonate (EMS), benzidine (BZD) and benzo[a]pyrene (B[a]P) were assayed for ability to induce mutation at the hprt locus of wild-type L5178Y mouse lymphoma cells. EMS was assayed in the absence of metabolic activation, B[a]P in the presence of metabolic activation (S-9 mix) and BZD both in the absence and presence of S-9. Treatment with EMS minus S-9 and B[a]P plus S-9, especially when the S-9 content of the incubation was 2% (v/v), produced strong dose-related increases in mutant frequency. BZD failed to induce mutation at the hprt locus, either in the absence or presence of S-9.
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Kennelly JC, Clare CB, Campbell J et al (1990) The mutagenic activity of ethylmethanesulphonate, benzidine and benzo[a]pyrene at the hprt locus of wild-type L5178Y mouse lymphoma cells. Mutagenesis. 5(Supplement): 21-26.
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