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An optimised assay for quantitative, high-throughput analysis of polysialyltransferase activity

Elkashef, Sara M.
; ; 
Patterson, Laurence H.
; ; 
Falconer, Robert A.
Publication Date
2016-08-08
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Keywords
Polysialytransferases, Glycosyltransferase enzymes, Polysialic acid, Carbohydrate polymers, Cancer, DMB-DP3 acceptor, Assay
Rights
© 2016 Royal Society of Chemistry. Full-text reproduced in accordance with the publisher's self-archiving policy.
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Peer-Reviewed
Yes
Open Access status
openAccess
Accepted for publication
2016-08-07
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Life Sciences Publications
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Abstract
The polysialyltransferases are biologically important glycosyltransferase enzymes responsible for the biosynthesis of polysialic acid, a carbohydrate polymer that plays a critical role in the progression of several diseases, notably cancer. Having improved the chemical synthesis and purification of the fluorescently-labelled DMB-DP3 acceptor, we report optimisation and validation of a highly sensitive cell-free high-throughput HPLC-based assay for assessment of human polysialyltransferase activity.
URI
http://hdl.handle.net/10454/8803
Version
Accepted manuscript
Citation
Elkashef SM, Sutherland M, Patterson LH, Loadman PM and Falconer RA (2016) An optimised assay for quantitative, high-throughput analysis of polysialyltransferase activity. Analyst. 141(20): 5849-5856.
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Link to Version of Record
https://doi.org/10.1039/C6AN01073C
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Article
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