Production of monoclonal antibodies to sugarcane yellow leaf virus using recombinant read-through protein.
Coates, David Danks, C. Korimbocus, J. Preston, S. Boonham, N. Barker, I.
Coates, David
Danks, C.
Korimbocus, J.
Preston, S.
Boonham, N.
Barker, I.
Publication Date
2009-07-21
End of Embargo
Supervisor
Rights
Peer-Reviewed
Yes
Open Access status
closedAccess
Accepted for publication
Institution
Department
Awarded
Embargo end date
Collections
Abstract
Yellow leaf syndrome (YLS) of sugarcane is associated with sugarcane yellow leaf virus (SCYLV), a member of the family Luteoviridae. A fragment of the coat protein and readthrough domain of SCYLV was expressed in a bacterial expression system. The resulting protein was purified and used to immunize mice for monoclonal antibody (MAb) production. Two MAbs, 3A2E3 and 2F7H5, were selected following the screening of hybridoma cells using both plate-trapped antigen enzyme-linked immunosorbent assay (PTA-ELISA) and tissue blot immunoassay (TBIA). These MAbs can be incorporated into the TBIA assay currently used for the routine detection of SCYLV but could not be used in triple antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA). The two antibodies selected have slightly different specificities. Antibody 3A2E3 gave equivalent results to a polyclonal antiserum (raised to purified virus) in comparative testing using TBIA. The MAbs produced should provide a widely available, uniform reagent for SCYLV diagnosis with the potential to help manage YLS.
Version
Published version
Citation
Coates, D, Danks, C., Korimbocus, J. and Preston S. et al. (2002). Production of monoclonal antibodies to sugarcane yellow leaf virus using recombinant read-through protein. Journal of Phytopathology. Vol. 150, No. 8-9, pp. 488-494.
Link to publisher’s version
Link to published version
Link to Version of Record
Type
Article