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What's the catch? Archaeological application of rapid collagen-based species identification for Pacific Salmon

Korzow Richter, K.
; 
McGrath, K.
; 
Masson-MacLean, E.
; 
Hickinbotham, S.
; ; 
Britton, K.
; 
Bottomley, Z.
; 
Dobney, K.
; 
Hulme-Beaman, A.
; 
Zona, M.
... show 3 more
Publication Date
2020-04
End of Embargo
Supervisor
Keywords
ZooMS, Collagen peptide, Mass fingerprinting, Pacific salmon, Yup'Ik, Nunalleq
Rights
© 2020 Elsevier. Reproduced in accordance with the publisher's self-archiving policy. This manuscript version is made available under the CC-BY-NC-ND 4.0 license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
cb
Peer-Reviewed
Yes
Open Access status
openAccess
Accepted for publication
2020-02-19
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Department
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Life Sciences Publications
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Abstract
Pacific salmon (Oncorhynchus spp.) are ecological and cultural keystone species along the Northwest Coast of North America and are ubiquitous in archaeological sites of the region. The inability to morphologically identify salmonid post-cranial remains to species, however, can limit our understanding of the ecological and cultural role different taxa played in the seasonal subsistence practices of Indigenous groups in the past. Here, we present a rapid, cost-effective ZooMS method to distinguish salmonid species based on collagen peptide mass-fingerprinting. Using modern reference material and an assemblage of 28 DNA-identified salmonid bones from the pre-contact Yup'ik site of Nunalleq, Western Alaska, we apply high-resolution mass spectrometry (LC-MS/MS) to identify a series of potential collagen peptide markers to distinguish Pacific salmon. We then confirm these peptide markers with a blind ZooMS analysis (MALDI-TOF-MS) of the archaeological remains. We successfully distinguish five species of anadromous salmon with this ZooMS approach, including one specimen that could not be identified through ancient DNA analysis. Our biomolecular identification of chum (43%), sockeye (21%), chinook (18%), coho (11%) and pink (7%), confirm the exploitation of all five available species of salmonid at Nunalleq.
URI
http://hdl.handle.net/10454/17771
Version
Accepted manuscript
Citation
Korzow Richter K, McGrath K, Masson-MacLean E et al (2020) What's the catch? Archaeological application of rapid collagen-based species identification for Pacific Salmon. Journal of Archaeological Science. 116: 105116.
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https://doi.org/10.1016/j.jas.2020.105116
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Article
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