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Equation to Line the Borders of the Folding–Unfolding Transition Diagram of Lysozyme
Mohammad, Mohammad A. Grimsey, Ian M. Forbes, Robert T.
Mohammad, Mohammad A.
Grimsey, Ian M.
Forbes, Robert T.
Publication Date
2016-07
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This document is the Accepted Manuscript version of a Published Work that appeared in final form in The Journal of Physical Chemistry B, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/acs.jpcb.6b01317
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openAccess
Accepted for publication
2016-06-24
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Abstract
It is important for the formulators of biopharmaceuticals to predict the folding–unfolding transition of proteins. This enables them to process proteins under predetermined conditions, without denaturation. Depending on the apparent denaturation temperature (Tm) of lysozyme, we have derived an equation describing its folding–unfolding transition diagram. According to the water content and temperature, this diagram was divided into three different areas, namely, the area of the water-folded lysozyme phase, the area of the water-folded lysozyme phase and the bulk water phase, and the area of the denatured lysozyme phase. The water content controlled the appearance and intensity of the Raman band at ∼1787 cm–1 when lysozyme powders were thermally denatured at temperatures higher than Tm.
Version
Accepted manuscript
Citation
Mohammad MA, Grimsey IM and Forbes RT (2016) Equation to line the borders of the folding-unfolding transition diagram of lysozyme. The Journal of Physical Chemistry B. 120(28): 6911-6916.
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Article