Optimal determination of steric mass action model parameters for beta-lactoglobulin using static batch experiments
Barz, T. Loffler, V. Arellano-Garcia, Harvey Wozny, G.
Barz, T.
Loffler, V.
Arellano-Garcia, Harvey
Wozny, G.
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2010
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Abstract
In this work, parameters of the steric mass-formalism SMA are optimally ascertained for a reliable determination of the adsorption isotherms of beta-lactoglobulin A and B under non-isocratic conditions. For this purpose, static batch experiments are used in contrast to the protocols based on different experimental steps, which use a chromatographic column. It is shown that parameters can already be determined for a small number of experiments by using a systematic procedure based on optimal model-based experimental design and an efficient NLP-solver. The in different works observed anti-Langmuir shape of the isotherm for small concentrations of beta-lactoglobulin A was corroborated. Moreover, we also found indications for a porosity variation with changing protein concentrations.
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Barz T, Loffler V, Arellano-Garcia H et al (2010) Optimal determination of steric mass action model parameters for beta-lactoglobulin using static batch experiments. Journal of Chromatography A. 1217(26): 4267-4277.
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