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A Method for Estimation of Plasma Protein Binding Using Diffusion Ordered NMR Spectroscopy (DOSY)

Taylor, Rachel
; 
Wilkinson, D.
; ; 
Publication Date
2024-07-01
End of Embargo
Supervisor
Keywords
Plasma protein binding, DOSY
Rights
(c) 2024 Royal Society of Chemistry. Full-text reproduced in accordance with the publisher's self-archiving policy.
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Peer-Reviewed
Yes
Open Access status
embargoedAccess
Accepted for publication
2024-06-06
Institution
Department
Awarded
Embargo end date
2025-06-11
Collections
Life Sciences Publications
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Additional title
Abstract
The plasma protein binding (PPB) of a drug plays a key role in both its pharmacokinetic and pharmacodynamic properties. During lead optimisation, medium and high throughput methods for the early determination of PPB can provide important information about potential PKPD profile within a chemotype or between different chemotype series. Diffusion ordered spectroscopy (DOSY) is an NMR spectroscopic technique that measures the diffusion of a molecule via applying varying magnetic field gradients, where the diffusion is primarily affected by its molecular size/weight and solution viscosity. Here, we describe the use of DOSY for a rapid and straight forward method to evaluate the PPB of drug molecules, using their binding to bovine serum albumin (BSA) as a model.
URI
http://hdl.handle.net/10454/19948
Version
Accepted manuscript
Citation
Taylor R, Wilkinson D, Swift T et al (2024) A Method for Estimation of Plasma Protein Binding Using Diffusion Ordered NMR Spectroscopy (DOSY) RSC Medicinal Chemistry. 7(15): 2372-2379.
Link to publisher’s version
Link to published version
Link to Version of Record
https://doi.org/10.1039/D4MD00244J
Type
Article
Qualification name
Notes
The full-text of this article will be released for public view at the end of the publisher embargo on 11 Jun 2025.