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Exploring a role for regulatory miRNAs in wound healing during ageing: involvement of miR-200c in wound repair
Aunin, Eerik Broadley, David Ahmed, Mohammed I. Mardaryev, Andrei N. Botchkareva, Natalia V.
Aunin, Eerik
Broadley, David
Ahmed, Mohammed I.
Mardaryev, Andrei N.
Botchkareva, Natalia V.
Publication Date
2017
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© 2017 The Authors. This article is licensed under a Creative Commons
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openAccess
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2017-04-26
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Abstract
Multiple factors and conditions can lead to impaired wound healing. Chronic non-healing wounds
are a common problem among the elderly. To identify microRNAs negatively impacting the wound
repair, global miRNA profiling of wounds collected from young and old mice was performed. A
subset of miRNAs that exhibited an age-dependent expression pattern during wound closure was
identified, including miR-31 and miR-200c. The expression of miR-200 family members was markedly
downregulated upon wounding in both young and aged mice, with an exception of acute
upregulation of miR-200c at the early phase of wound healing in aged skin. In unwounded aged skin
(versus unwounded younger skin), the level of miR-200c was also found elevated in both human and
mice. Overexpression of miR-200c in human ex vivo wounds delayed re-epithelialisation and
inhibited cell proliferation in the wound epithelium. Modulation of miR-200c expression in both
human and mouse keratinocytes in vitro revealed inhibitory effects of miR-200c on migration, but
not proliferation. Accelerated wound closure in vitro induced by anti-miR-200c was associated with
upregulation of genes controlling cell migration. Thus, our study identified miR-200c as a critical
determinant that inhibits cell migration during skin repair after injury and may contribute to ageassociated
alterations in wound repair.
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Citation
Aunin E, Broadley DP, Ahmed MI et al (2017) Exploring a role for regulatory miRNAs in
wound healing during ageing: involvement of miR-200c in wound repair. Scientific Reports. 7:
Article number 3257.
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Article