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Exploring a role for regulatory miRNAs in wound healing during ageing: involvement of miR-200c in wound repair
Aunin, Eerik ; Broadley, David ; Ahmed, Mohammed I. ; Mardaryev, Andrei N. ; Botchkareva, Natalia V.
Aunin, Eerik
Broadley, David
Ahmed, Mohammed I.
Mardaryev, Andrei N.
Botchkareva, Natalia V.
Publication Date
2017
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© 2017 The Authors. This article is licensed under a Creative Commons
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openAccess
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2017-04-26
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Abstract
Multiple factors and conditions can lead to impaired wound healing. Chronic non-healing wounds
are a common problem among the elderly. To identify microRNAs negatively impacting the wound
repair, global miRNA profiling of wounds collected from young and old mice was performed. A
subset of miRNAs that exhibited an age-dependent expression pattern during wound closure was
identified, including miR-31 and miR-200c. The expression of miR-200 family members was markedly
downregulated upon wounding in both young and aged mice, with an exception of acute
upregulation of miR-200c at the early phase of wound healing in aged skin. In unwounded aged skin
(versus unwounded younger skin), the level of miR-200c was also found elevated in both human and
mice. Overexpression of miR-200c in human ex vivo wounds delayed re-epithelialisation and
inhibited cell proliferation in the wound epithelium. Modulation of miR-200c expression in both
human and mouse keratinocytes in vitro revealed inhibitory effects of miR-200c on migration, but
not proliferation. Accelerated wound closure in vitro induced by anti-miR-200c was associated with
upregulation of genes controlling cell migration. Thus, our study identified miR-200c as a critical
determinant that inhibits cell migration during skin repair after injury and may contribute to ageassociated
alterations in wound repair.
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Citation
Aunin E, Broadley DP, Ahmed MI et al (2017) Exploring a role for regulatory miRNAs in
wound healing during ageing: involvement of miR-200c in wound repair. Scientific Reports. 7:
Article number 3257.
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