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Self-assembled carrageenan/protamine polyelectrolyte nanoplexes-Investigation of critical parameters governing their formation and characteristics

Dul, M.
; 
Paluch, Krzysztof J.
; 
Kelly, H.
; 
Healy, A.M.
; 
Sasse, A.
; 
Tajber, L.
Publication Date
05/06/2015
End of Embargo
Supervisor
Keywords
Calcium, Carrageenan, Electrolytes, Ions, Microscopy, Nanoparticles, Particle size, Protamines, Spectroscopy, Fourier transform infrared, Zinc, Dynamic light scattering, Infrared spectroscopy, Polyelectrolyte complexes
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Peer-Reviewed
Yes
Open Access status
openAccess
Accepted for publication
29/01/2015
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Life Sciences Publications
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Abstract
The aim of this work was to investigate the feasibility of cross-linker free polyelectrolyte complex formation at the nanoscale between carrageenan (CAR) and protamine (PROT). The properties of CAR/PROT nanoparticles (NPs) were dependent on the carrageenan type: kappa (KC), iota (IC) and lambda (LC), concentration of components, addition of divalent cations, weight mixing ratio (WMR) of constituents and mode of component addition. In the case of 0.1% w/v solutions, IC-based NPs had the smallest particle sizes (100-150nm) and low polydispersity indices (0.1-0.4). A decrease in the solution concentration from 0.1% to 0.05% w/v enabled the formation of KC/PROT NPs. All carrageenans exhibited the ability to form NPs with surface charge ranging from -190 to 40mV. The inclusion of divalent cations caused an increase in the particle size and zeta potential. Infrared analysis confirmed the presence of a complex between CAR and PROT and showed that IC chains undergo structural changes when forming NPs. Colloidal stability of NPs was related to the initial surface charge of particles and was time- and pH-dependent. IC was found to be the most suitable type of CAR when forming nanoplexes with PROT.
URI
http://hdl.handle.net/10454/9334
Version
Accepted manuscript
Citation
Dul M, Paluch KJ, Kelly H, Healy AM, Sasse A and Tajber L (2015) Self-assembled carrageenan/protamine polyelectrolyte nanoplexes-Investigation of critical parameters governing their formation and characteristics. Carbohydrate Polymers. 123: 339-349.
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https://doi.org/10.1016/j.carbpol.2015.01.066
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Article
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