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Development and Validation of Micro Emulsion High Performance Liquid Chromatography(MELC) Method for the Determination of Nifedipine in Pharmaceutical Preparation
Al-Jammal, M.K.H. Al Ayoub, Yuosef Assi, Khaled H.
Al-Jammal, M.K.H.
Al Ayoub, Yuosef
Assi, Khaled H.
Publication Date
2015-03-03
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© 2015 Al-Jammal MKH, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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2015-02-24
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Abstract
Microemulsion is a stable, isotropic clear solution consisting of oil based substance, water surfactant and cosurfactant.
There are two types of microemulsion which are used as a mobile phase; water in oil (w/o) and oil in water
(o/w).Microemulsion has a strong ability to solubilize both hydrophobic and hydrophilic analytes, therefore reducing
the pre-treatment of the sample which is needed for the complex sample. Recent reports found that separating the
analytes by using microemulsion high performance liquid chromatography can be achieved with superior speed and
efficiency compared to conventional HPLC modes. In this work, Oil in water (o/w) microemulsion has been used
for the determination of nifedipine in pharmaceutical preparation. The effect of each parameter on the separation
process was examined. The samples were injected into C18, analytical columns maintained at 30°C with a flow rate 1
ml/min. The mobile phase was 87.1% aqueous orthophosphate buffer 15 mM (adjusted to pH 3 with orthophosphoric
acid), 0.8% of octane as oil, 4.5 SDS, and 7.6% 1-butanol, all w/w. The nifedipine and internal standard peaks were
detected by UV detection at λ max 237 nm
The calibration curve was linear (r2=0.9995) over nifedipine concentrations ranging from 1 to 60 μg/ml (n=6). The
method has good sensitivity with limit of detection (LOD) of 0.33 μg/ml and limit of quantitation (LOQ) of 1.005 μg/
ml. Also it has an excellent accuracy ranging from 99.11 to 101.64%. The intra-day and inter-day precisions (RSD
%) were <0.45% and <0.9%, respectively.
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Citation
Al-Jammal MKH, Al Ayoub Y, Assi KH (2015) Development and Validation of Micro Emulsion High Performance Liquid Chromatography(MELC) Method for the Determination of Nifedipine in Pharmaceutical Preparation. Pharmeutica Analytica Acta. 6(3)
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