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Development of a Basement Membrane Substitute Incorporated Into an Electrospun Scaffold for 3D Skin Tissue Engineering
Bye, F.J. Bullock, A.J. Singh, R. Sefat, Farshid Roman, S. MacNeil, S.
Bye, F.J.
Bullock, A.J.
Singh, R.
Sefat, Farshid
Roman, S.
MacNeil, S.
Publication Date
2014
End of Embargo
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© 2014 American Scientific Publishers. Reproduced with the permission of the publisher.
Peer-Reviewed
Yes
Open Access status
openAccess
Accepted for publication
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Abstract
A major challenge in the production of 3D tissue engineered skin is the recreation of the basement
membrane region to promote secure attachment and yet segregation of keratinocytes from
the dermal substitute impregnated with fibroblasts. We have previously shown that simple electrospun
scaffolds provide fibres on which the cells attach, proliferate, and self-sort into epithelium and
dermis. In a development of this in this study tri-layered scaffolds were then electrospun from poly
L-lactic acid and poly hydroxybutyrate-co-hydroxyvalerate. In these a central layer of the scaffolds
comprising nano-porous/nano-fibrous poly hydroxybutyrate-co-hydroxyvalerate fibres was interwoven
into the bulk micro-porous poly L-lactic acid microfibers to mimic the basement membrane.
Keratinocytes and fibroblasts seeded onto these scaffolds and cultured for 2 weeks showed that
neither cell type was able to cross the central nano-porous barrier (shown by SEM, and fluorescence
monitoring with CellTrackerâ„¢) while the micro-fibrous poly L-lactic acid provided a scaffold
on which keratinocytes could create an epithelium and fibroblasts could create a dermal substitute
depositing collagen. Although cells did not penetrate this barrier the interaction of cells was still
evident-essential for epithelial development.
Version
Accepted manuscript
Citation
Bye FJ, Bullock AJ, Singh R, Sefat F, Roman S and MacNeil S (2014) Development of a basement membrane substitute incorporated into an electrospun scaffold for 3D skin tissue engineering. Journal of Biomaterials and Tissue Engineering. 4: 1-7.
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Article