The role of endothelial cell reactive antibodies in peripheral arterial disease
Lindsey, Nigel J. Armitage, J.D. Homer-Vanniasinkam, Shervanthi
Lindsey, Nigel J.
Armitage, J.D.
Homer-Vanniasinkam, Shervanthi
Publication Date
2006
End of Embargo
Supervisor
Rights
Peer-Reviewed
Yes
Open Access status
closedAccess
Accepted for publication
Institution
Department
Awarded
Embargo end date
Collections
Additional title
Abstract
Objectives
It is hypothesised that endothelial cell reactive antibodies (ECRA) play a role in the progression of PAD through activation of endothelial cells and the release of inflammatory cytokines. We aimed to test this hypothesis by assessing levels of ECRA, E-selectin and IL-6 in patients with PAD of varying severity in a case controlled study.
Design, materials, methods
Patients were assessed clinically and with ankle¿brachial pressure indices. Patients with critical ischaemia (CI, n=30), stable claudicants (SC, n=30), and age-matched controls (AMC, n=20) were studied. Antibody, E-selectin and IL-6 levels were measured using ELISA.
Results
ECRA levels were significantly raised in the CI group over AMC.
IL-6 levels were significantly elevated in both SC and CI over the control group and in CI over SC.
There were no significant differences in E-selectin levels between the AMC, SC and CI.
Conclusion
Our findings support the hypothesis that autoantibodies play a role in promoting PAD by elevating IL-6. The absence of an elevation in E-selectin in this study may be due to its short half-life, and casts doubt on its value as a marker of inflammation in atherosclerosis.
Version
No full-text in the repository
Citation
Lindsey, N.J., Armitage, J.D. and Horner-Vanniasinkam S. (2006). The role of endothelial cell reactive antibodies in peripheral arterial disease. European Journal of Vascular and Endovascular Surgery. Vol. 31, No. 2, pp. 170-175.
Link to publisher’s version
Link to published version
Link to Version of Record
Type
Article