Publication

Novel and established potassium channel openers stimulate hair growth in vitromodes of action in hair follicles.: implications for their

Davies, Gareth C.
; 
Thornton, M. Julie
; 
Jenner, Tracey J.
; 
Chen, Yi-Ju
; 
Hansen, J.B.
; 
Carr, R.D.
; 
Randall, Valerie A.
Publication Date
2005
End of Embargo
Supervisor
Keywords
Bioassay, Diazoxide, Hair follicles, Glibenclamide, Minoxidil, NNC 55-0118, Organ culture, Phenol red, Tolbutamide
Rights
Peer-Reviewed
Yes
Open Access status
closedAccess
Accepted for publication
Institution
Department
Awarded
Embargo end date
Collections
Life Sciences Publications
Show all metadata
Additional title
Abstract
Although ATP-sensitive potassium (K(ATP)) channel openers, e.g., minoxidil and diazoxide, can induce hair growth, their mechanisms require clarification. Improved drugs are needed clinically. but the absence of a good bioassay hampers research. K(ATP) channels from various tissues contain subtypes of the regulatory sulfonylurea receptor, SUR, and pore-forming, K(+) inward rectifier subunits, Kir6.X, giving differing sensitivities to regulators. Therefore, the in vitro effects of established potassium channel openers and inhibitors (tolbutamide and glibenclamide), plus a novel, selective Kir6.2/SUR1 opener, NNC 55-0118, were assessed on deer hair follicle growth in serum-free median without streptomycin. Minoxidil (0.1-100 microM, p<0.001), NNC 55-0118 (1 mM, p<0.01; 0.1, 10, 100 microM, p<0.001), and diazoxide (10 microM, p<0.01) increased growth. Tolbutamide (1 mM) inhibited growth (p<0.001) and abolished the effect of 10 microM minoxidil, diazoxide and NNC 55-0118; glibenclamide (10 microM) had no effect, but prevented stimulation by 10 microM minoxidil. Phenol red stimulated growth (p<0.001), but channel modulator responses remained unaltered. Thus, deer follicles offer a practical, ethically advantageous in vitro bioassay that reflects clinical responses in vivo. The results indicate direct actions of K(ATP) channel modulators within hair follicles via two types of channels, with SUR 1 and SUR 2, probably SUR2B, sulfonylurea receptors.
URI
http://hdl.handle.net/10454/4017
Version
No full-text in the repository
Citation
Davies, G.C., Thornto, M.J., Jenner, T.J. and Randall, V.A. et al. (2005). Novel and established potassium channel openers stimulate hair growth in vitro: implications for their modes of action in hair follicles. Journal of Investigative Dermatology. Vol. 124, No. 4, pp. 686-694.
Link to publisher’s version
Link to published version
Link to Version of Record
https://doi.org/10.1111/j.0022-202X.2005.23643.x
Type
Article
Qualification name
Notes