Loading...
ROS-induced Oxidative Damage in Lymphocytes Ex Vivo/in Vitro From Healthy Individuals and MGUS Patients: Protection by Myricetin Bulk and Nanoforms
Akhtar, Shabana Najafzadeh, Mojgan Isreb, Mohammad Newton, L. Gopalan, Rajendran C. Anderson, Diana
Akhtar, Shabana
Najafzadeh, Mojgan
Isreb, Mohammad
Newton, L.
Gopalan, Rajendran C.
Anderson, Diana
Publication Date
2020-04
End of Embargo
Supervisor
Rights
(c) 2020 The Authors. This is an Open Access article distributed under the Creative Commons CC-BY license (http://creativecommons.org/licenses/by/4.0/)
Peer-Reviewed
Yes
Open Access status
openAccess
Accepted for publication
2020-02-21
Institution
Department
Awarded
Embargo end date
Collections
Additional title
Abstract
We investigated the protective role of myricetin bulk and nanoforms, against reactive oxygen species (ROS)-induced oxidative stress caused by hydrogen peroxide and tertiary-butyl hydro peroxide in lymphocytes in vitro from healthy individuals and those from pre-cancerous patients suffering with monoclonal gammopathy of undetermined significance (MGUS). The change in intracellular reactive oxygen species was measured once cells were treated with myricetin bulk forms and nanoforms with and without either hydrogen peroxide or tertiary-butyl hydro peroxide co-supplementation. The direct and indirect antioxidant activity of myricetin was spectrofluometrically measured using the fluorescent dye 2',7'-dichlorofluorescin diacetate and using the Comet assay, respectively. Hydrogen peroxide (50 µM) and tertiary-butyl hydro peroxide (300 µM) induced a higher level of reactive oxygen species-related DNA damage and strand breaks. Addition of myricetin nanoform (20 µM) and bulk (10 µM) form could, however, significantly prevent hydrogen peroxide- and tertiary-butyl hydro peroxide-induced oxidative imbalances and the nanoform was more effective. Glutathione levels were also quantified using a non-fluorescent dye. Results suggest that myricetin treatment had no significant effect on the cellular antioxidant enzyme, glutathione. The current study also investigates the effect of myricetin on the induction of double-strand breaks by staining the gamma-H2AX foci immunocytochemically. It was observed that myricetin does not induce double-strand breaks at basal levels rather demonstrated a protective effect.
Version
Published version
Citation
Akhtar S, Najafzadeh M, Isreb M et al (2020) ROS-induced oxidative damage in lymphocytes ex vivo/in vitro from healthy individuals and MGUS patients: protection by myricetin bulk and nanoforms. Archives of Toxicology. 94: 1229-1239.
Link to publisher’s version
Link to published version
Link to Version of Record
Type
Article