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Identifying molecular mass of coagulant protein from edible Hibiscus seeds using SDS-PAGE analysis
Jones, Alfred N. Bridgeman, John
Jones, Alfred N.
Bridgeman, John
Publication Date
2019-03
End of Embargo
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(c) 2019 ASCE. This material may be downloaded for personal use only. Any other use requires prior permission of the American Society of Civil Engineers. This material may be found at https://doi.org/10.1061/(ASCE)EE.1943-7870.0001595.
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Yes
Open Access status
openAccess
Accepted for publication
2019-03
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Abstract
This study used sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis and a jar test apparatus to investigate the molecular weight (MW) and turbidity removal potential of Hibiscus seeds. Three Hibiscus species were assessed: okra crude extract (OCE), sabdariffa crude extract (SCE), and kenaf crude extract (KCE). Furthermore, purified versions of each [i.e., purified okra protein (POP), purified sabdariffa protein (PSP), and purified kenaf protein (PKP)] obtained from anionic exchange were evaluated. The results show that whereas the crude extracts had multiple proteins with MW sizes between 11 and 82 kDa, the purified samples consisted of a single coagulant protein band around 39 kDa. In each case, significant turbidity removal was recorded with the purified proteins; POP, PSP and PKP achieved approximately 98%, 94%, and 90% removal, respectively, at a reduced dosage of ≤0.6 mg/L. However, OCE and SCE achieved lower turbidity removal of 86% and 85% using 40-mg/L doses, respectively, whereas KCE recorded only 73% turbidity removal with a 60-mg/L dose. Sludge generation by crude and purified proteins was approximately 25% of sludge produced by aluminum sulfate and had the additional benefit of being biodegradable. Therefore, the coagulant protein in Hibiscus plant seeds has potential applications for improvements to accessing clean water in developing countries.
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Accepted manuscript
Citation
Jones AN and Bridgeman J (2019) Identifying molecular mass of coagulant protein from edible Hibiscus seeds using SDS-PAGE analysis. Journal of the Environmental Engineering Division. 145(11)
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Article