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Genetic basis and timing of a major mating system shift in Capsella
Bachmann, J.A. Laenen, B. Fracassetti, M. Désamoré, A. Lafon-Placette, C. Steige, K.A. Callot, C. Marande, W. Neuffer, B.
Bachmann, J.A.
Laenen, B.
Fracassetti, M.
Désamoré, A.
Lafon-Placette, C.
Steige, K.A.
Callot, C.
Marande, W.
Neuffer, B.
Publication Date
2019-06
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© 2019 The Authors New Phytologist. © 2019 New Phytologist Trust. This is the peer reviewed version of the following article: Bachmann JA, Tedder A, Laenen B et al (2019) Genetic basis and timing of a major mating system shift in Capsella. New Phytologist, 224: 505-517, which has been published in final form at https://doi.org/10.1111/nph.16035. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
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2019-06-20
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Abstract
A crucial step in the transition from outcrossing to self-fertilization is the loss of genetic self-incompatibility (SI). In the Brassicaceae, SI involves the interaction of female and male speci-ficity components, encoded by the genesSRKandSCRat the self-incompatibility locus (S-lo-cus). Theory predicts thatS-linked mutations, and especially dominant mutations inSCR, arelikely to contribute to loss of SI. However, few studies have investigated the contribution ofdominant mutations to loss of SI in wild plant species. Here, we investigate the genetic basis of loss of SI in the self-fertilizing crucifer speciesCapsella orientalis, by combining genetic mapping, long-read sequencing of completeS-hap-lotypes, gene expression analyses and controlled crosses. We show that loss of SI inC. orientalisoccurred<2.6 Mya and maps as a dominant trait totheS-locus. We identify a fixed frameshift deletion in the male specificity geneSCRand con-firm loss of male SI specificity. We further identify anS-linked small RNA that is predicted tocause dominance of self-compatibility. Our results agree with predictions on the contribution of dominantS-linked mutations toloss of SI, and thus provide new insights into the molecular basis of mating system transitions.
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Bachmann JA, Tedder A, Laenen B et al (2019) Genetic basis and timing of a major mating system shift in Capsella. New Phytologist, 224: 505-517.
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