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Trichohyalin is a potential major autoantigen in human alopecia areata

Leung, Man Ching
; 
Sutton, Chris W.
; 
Fenton, D.A.
; 
Tobin, Desmond J.
Publication Date
2010
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Keywords
Adult, Alopecia Areata, Blood, Immunology, Autoantigens, Analysis, Female, Fluorescent antibody technique, Indirect, Hair follicle, Pathology, Humans, Intermediate filament proteins, Keratin-16, Male, Protein precursors, Spectrometry, Mass, Matrix-assisted laser desorption-Ionization, REF 2014
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Abstract
Several lines of evidence support an autoimmune basis for alopecia areata (AA), a common putative autoimmune hair loss disorder. However, definitive support is lacking largely because the identity of hair follicle (HF) autoantigen(s) involved in its pathogenesis remains unknown. Here, we isolated AA-reactive HF-specific antigens from normal human scalp anagen HF extracts by immunoprecipitation using serum antibodies from 10 AA patients. Samples were analyzed by LC-MALDI-TOF/TOF mass spectrometry, which indicated strong reactivity to the hair growth phase-specific structural protein trichohyalin in all AA sera. Keratin 16 (K16) was also identified as another potential AA-relevant target HF antigen. Double immunofluorescence studies using AA (and control sera) together with a monoclonal antibody to trichohyalin revealed that AA sera contained immunoreactivity that colocalized with trichohyalin in the growth phase-specific inner root sheath of HF. Furthermore, a partial colocalization of AA serum reactivity with anti-K16 antibody was observed in the outer root sheath of the HF. In summary, this study supports the involvement of an immune response to anagen-specific HFs antigens in AA and specifically suggests that an immune response to trichohyalin and K16 may have a role in the pathogenesis of the enigmatic disorder.
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http://hdl.handle.net/10454/6066
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Leung, M. C., Sutton, C. W., Fenton, D. A., Tobin, D. J. (2010) Trichohyalin is a potential major autoantigen in human alopecia areata. J Proteome Res, 9 (10), 5153-63.
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https://doi.org/10.1021/pr100422u
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