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Study of the chemotactic response of multicellular spheroids in a microfluidic device
Ayuso, J.M. Basheer, Haneen A. Monge, R. Sánchez-Álvarez, P. Doblare, M. Vinader, Victoria Fernandez, L.J. Ochoa, I.
Ayuso, J.M.
Basheer, Haneen A.
Monge, R.
Sánchez-Álvarez, P.
Doblare, M.
Vinader, Victoria
Fernandez, L.J.
Ochoa, I.
Publication Date
2015-10-07
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© 2015 Ayuso et al. This is an open access article distributed under the
terms of the Creative Commons Attribution License, which permits unrestricted use, distribution,
and reproduction in any medium, provided the original author and source are credited.
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openAccess
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2015-09-13
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Abstract
We report the first application of a microfluidic device to observe chemotactic migration in
multicellular spheroids. A microfluidic device was designed comprising a central microchamber
and two lateral channels through which reagents can be introduced. Multicellular
spheroids were embedded in collagen and introduced to the microchamber. A gradient of
fetal bovine serum (FBS) was established across the central chamber by addition of growth
media containing serum into one of the lateral channels. We observe that spheroids of oral
squamous carcinoma cells OSC–19 invade collectively in the direction of the gradient of
FBS. This invasion is more directional and aggressive than that observed for individual cells
in the same experimental setup. In contrast to spheroids of OSC–19, U87-MG multicellular
spheroids migrate as individual cells. A study of the exposure of spheroids to the chemoattractant
shows that the rate of diffusion into the spheroid is slow and thus, the chemoattractant
wave engulfs the spheroid before diffusing through it.
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Published version
Citation
Ayuso JM, Basheer HA, Monge R et al (2015) Study of the chemotactic response of
multicellular spheroids in a microfluidic device. PLoS ONE. 10(10): e0139515.
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Article