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Proteomics of tissue factor silencing in cardiomyocytic cells reveals a new role for this coagulation factor in splicing machinery control

Lento, S.
; 
Brioschi, M.
; 
Barcella, S.
; 
Nasim, Md. Talat
; 
Ghilardi, S.
; 
Barbieri, S.S.
; 
Tremoli, E.
; 
Banfi, C.
Publication Date
24/04/2015
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Keywords
Tissue factor, Proteomics, Spliceosome, Splicing, Cardiomyocytic cells, Label-free quantitative mass spectrometry
Rights
© 2015 Elsevier B.V. Full-text reproduced in accordance with the publisher's self-archiving policy. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
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Yes
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openAccess
Accepted for publication
25/01/2015
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Life Sciences Publications
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Abstract
It has long been known that Tissue Factor (TF) plays a role in blood coagulation and has a direct thrombotic action that is closely related to cardiovascular risk, but it is becoming increasingly clear that it has a much wider range of biological functions that range from inflammation to immunity. It is also involved in maintaining heart haemostasis and structure, and the observation that it is down-regulated in the myocardium of patients with dilated cardiomyopathy suggests that it influences cell-to-cell contact stability and contractility, and thus contributes to cardiac dysfunction. However, the molecular mechanisms underlying these coagulation-independent functions have not yet been fully elucidated. In order to analyse the influence of TF on the cardiomyocitic proteome, we used functional biochemical approaches incorporating label-free quantitative proteomics and gene silencing, and found that this provided a powerful means of identifying a new role for TF in regulating splicing machinery together with the expression of several proteins of the spliceosome, and mRNA metabolism with a considerable impact on cell viability.
URI
http://hdl.handle.net/10454/8001
Version
Accepted manuscript
Citation
Lento S, Brioschi M, Barcella S, Nasim MT et al (2015) Proteomics of tissue factor silencing in cardiomyocytic cells reveals a new role for this coagulation factor in splicing machinery control. Journal of Proteomics. 119: 75-89.
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https://doi.org/10.1016/j.jprot.2015.01.021
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Article
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