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Effect of Transforming Growth Factor-β3 on mono and multilayer chondrocytes
Sefat, Farshid Khaghani, Seyed A. Soon, Chin Fhong Javid, Farideh A.
Sefat, Farshid
Khaghani, Seyed A.
Soon, Chin Fhong
Javid, Farideh A.
Publication Date
2016-07
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© 2016 Elsevier, Ltd. Reproduced in accordance with the publisher's self-archiving policy. This manuscript version is made available under the CC-BY-NC-ND 4.0 license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
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Yes
Open Access status
Accepted for publication
2016-04-18
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Abstract
Articular cartilage is an avascular and flexible connective tissue found in joints. It produces a cushioning effect at the joints and provides low friction to protect the ends of the bones from wear and tear/damage. It has poor repair capacity and any injury can result pain and loss of mobility. Transforming growth factor-beta (TGF-β), a cytokine superfamily, regulates cell function, including differentiation and proliferation. Although the function of the TGF-βs in various cell types has been investigated, their function in cartilage repair is as yet not fully understood. The effect of TGF-β3 in biological regulation of primary chondrocyte was investigated in this work. TGF-β3 provided fibroblastic morphology to chondrocytes and therefore overall reduction in cell proliferation was observed. The length of the cells supplemented with TGF-β3 were larger than the cells without TGF-β3 treatment. This was caused by the fibroblast like cells (dedifferentiated chondrocytes) which occupied larger areas compared to cells without TGF-β3 addition. The healing process of the model wound closure assay of chondrocyte multilayer was slowed down by TGF-β3, and this cytokine negatively affected the strength of chondrocyte adhesion to the cell culture surface.
Version
Accepted manuscript
Citation
Sefat F, Youseffi M, Khaghani SA, Soon CF and Javid F (2016) Effect of Transforming Growth Factor-β3 on mono and multilayer chondrocytes. Cytokine. 83: 118-126.
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Article