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Physicochemical and antibacterial characterization of a novel fluorapatite coating
Alhilou, A. Do, T. Mizban, L. Clarkson, B.H. Wood, David J.
Alhilou, A.
Do, T.
Mizban, L.
Clarkson, B.H.
Wood, David J.
Publication Date
2016-08
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© 2016 American Chemical Society.
ACS AuthorChoice - This is an open access article published under a Creative Commons
Attribution (CC-BY) License, which permits unrestricted use, distribution and reproduction in any
medium, provided the author and source are cited.
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Yes
Open Access status
openAccess
Accepted for publication
01/08/2016
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Abstract
Peri-implantitis remains the major impediment
to the long-term use of dental implants. With increasing
concern over the growth in antibiotic resistance, there is
considerable interest in the preparation of antimicrobial dental
implant coatings that also induce osseointegration. One such
potential coating material is fluorapatite (FA). The aim of this
study was to relate the antibacterial effectiveness of FA
coatings against pathogens implicated in peri-implantitis to the
physicochemical properties of the coating. Ordered and
disordered FA coatings were produced on the under and
upper surfaces of stainless steel (SS) discs, respectively, using a
hydrothermal method. Surface charge, surface roughness,
wettability, and fluoride release were measured for each
coating. Surface chemistry was assessed using X-ray photoelectron spectroscopy and FA crystallinity using X-ray diffraction.
Antibacterial activity against periodontopathogens was assessed in vitro using viable counts, confocal microscopy, and scanning
electron microscopy (SEM). SEM showed that the hydrothermal method produced FA coatings that were predominately aligned
perpendicular to the SS substrate or disordered FA coatings consisting of randomly aligned rodlike crystals. Both FA coatings
significantly reduced the growth of all examined bacterial strains in comparison to the control. The FA coatings, especially the
disordered ones, presented significantly lower charge, greater roughness, and higher area when compared to the control,
enhancing bacteria−material interactions and therefore bacterial deactivation by fluoride ions. The ordered FA layer reduced not
only bacterial viability but adhesion too. The ordered FA crystals produced as a potential novel implant coating showed
significant antibacterial activity against bacteria implicated in peri-implantitis, which could be explained by a detailed
understanding of their physicochemical properties.
Version
Published version
Citation
Alhilou A, Do T, Mizban L et al (2016) Physicochemical and antibacterial
characterization of a novel fluorapatite coating. ACS Omega. 1(2): 264-276.
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Article