Publication

MMP-10 is overexpressed, proteolytically active and a potential target for therapeutic intervention in human lung carcinomas

Gill, Jason H.
; 
Kirwan, Ian G.
; 
Seargent, Jill M.
; 
Martin, Sandie W.
; 
Tijani, S.
; 
Anikin, V.A.
; 
Mearns, A.J.
; 
Bibby, Michael C.
; 
Anthoney, Alan
; 
Publication Date
2004
End of Embargo
Supervisor
Keywords
Tumour Markers, Heterologous, Transplantation, Animals, Carcinoma, Cell line, Tumour, Enzyme activation, Immunohistochemistry, Lung neoplasms, Humans, Matrix Metalloproteinase 10, Metalloendopeptidases, Mice, Biological analysis
Rights
Peer-Reviewed
Yes
Open Access status
closedAccess
Accepted for publication
Institution
Department
Awarded
Embargo end date
Collections
Life Sciences Publications
Show all metadata
Additional title
Abstract
Matrix metalloproteinase (MMP)-mediated degradation of the extracellular matrix is a major factor for tumor development and expansion. This study analysed MMP-10 protein expression and activity in human lung tumors of various grade, stage, and type to address the relationship between MMP-10 and tumor characteristics and to evaluate MMP-10 as a therapeutic target in non small cell lung carcinoma (NSCLC). Unlike the majority of MMPs, MMP-10 was located in the tumor mass as opposed to tumor stroma. MMP-10 protein was observed at low levels in normal human lung tissues and at significantly higher levels in all types of NSCLC. No correlation was observed between MMP-10 protein expression and tumor type, stage, or lymph node invasion. To discriminate between active and inactive forms of MMP-10 in samples of human NSCLC, we have developed an ex vivo fluorescent assay. Measurable MMP-10 activity was detected in 42 of 50 specimens of lung cancer and only 2 of 10 specimens of histologically normal lung tissue. No relationship was observed between MMP-10 activity levels and clinicopathologic characteristics. Our results suggest that MMP-10 is expressed and active at high levels in human NSCLC compared to normal lung tissues, and, as such, is a potential target for the development of novel therapeutics for lung cancer treatment.
URI
http://hdl.handle.net/10454/4121
Version
No full-text in the repository
Citation
Gill JH, Kirwan IG, Seargent JM et al (2004) MMP-10 is overexpressed, proteolytically active and a potential target for therapeutic intervention in human lung carcinomas. Neoplasia. 6(6): 777-785.
Link to publisher’s version
Link to published version
Link to Version of Record
https://doi.org/10.1593/neo.04283
Type
Article
Qualification name
Notes