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Interaction of Acinetobacter sp. RIT 592 induces the production of broad-spectrum antibiotics in Exiguobacterium sp. RIT 594

; 
Miranda, R.R.
; 
Bedore, T.J.
; 
Watts, L.M.
; 
Mantravadi, P.K.
; 
Wong, N.H.
; 
Chu, J.
; 
Adjei, J.A.
; 
Rana, A.P.
; 
Savka, M.A.
... show 3 more
Publication Date
2024-08-01
End of Embargo
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Keywords
Antibiotics, AMR, MDR, Bioprospecting, GNPs, Antimicrobial peptides, Exiguobacterium, Acinetobacter
Rights
© 2024 Parthasarathy, Miranda, Bedore, Watts, Mantravadi, Wong, Chu, Adjei, Rana, Savka, Bulman, Borrego and Hudson. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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Peer-Reviewed
Yes
Open Access status
openAccess
Accepted for publication
2024-07-18
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Life Sciences Publications
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Parthasarathy_et_al_Frontiers_in_Pharmacology
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Abstract
Antimicrobial resistance (AMR) is one of the most alarming global public health challenges of the 21st century. Over 3 million antimicrobial-resistant infections occur in the United States annually, with nearly 50,000 cases being fatal. Innovations in drug discovery methods and platforms are crucial to identify novel antibiotics to combat AMR. We present the isolation and characterization of potentially novel antibiotic lead compounds produced by the cross-feeding of two rhizosphere bacteria, Acinetobacter sp. RIT 592 and Exiguobacterium sp. RIT 594. We used solid-phase extraction (SPE) followed by liquid chromatography (LC) to enrich antibiotic extracts and subsequently mass spectrometry (MS) analysis of collected fractions for compound structure identification and characterization. The MS data were processed through the Global Natural Product Social Molecular Networking (GNPS) database. The supernatant from RIT 592 induced RIT 594 to produce a cocktail of antimicrobial compounds active against Gram-positive and negative bacteria. The GNPS analysis indicated compounds with known antimicrobial activity in the bioactive samples, including oligopeptides and their derivatives. This work emphasizes the utility of microbial community-based platforms to discover novel clinically relevant secondary metabolites. Future work includes further structural characterization and antibiotic activity evaluation of the individual compounds against pathogenic multidrug-resistant (MDR) bacteria.
URI
http://hdl.handle.net/10454/20170
Version
Published version
Citation
Parthasarathy A, Miranda RR, Bedore TJ et al (2024) Interaction of Acinetobacter sp. RIT 592 induces the production of broad-spectrum antibiotics in Exiguobacterium sp. RIT 594. Frontiers in Pharmacology. 15: 1456027.
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Link to Version of Record
https://doi.org/10.3389/fphar.2024.1456027
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Article
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