The Effect of Transforming Growth Factor Beta (TGF-β3) and Sanicle on Wound Healing.
Beggs, Clive B. Denyer, Morgan C.T. Lemmerz, A. Sefat, Farshid Wright, Colin W.
Beggs, Clive B.
Denyer, Morgan C.T.
Lemmerz, A.
Sefat, Farshid
Wright, Colin W.
Publication Date
2010-07
End of Embargo
Supervisor
Rights
Peer-Reviewed
No
Open Access status
closedAccess
Accepted for publication
15/03/2010
Institution
Department
Awarded
Embargo end date
Collections
Additional title
Abstract
There is evidence that both the herb Sanicle and the
cytokine TGF- β3 can be beneficial in enhancing wound repair.
In this study 3T3 fibroblast cells were cultured and the
confluent monolayers were wounded (scarred) using a
disposable plastic pipette. Various amounts of TGF-β3 (a
growth factor) and Sanicle extract were applied to the cell
monolayers. TGF-β3 was applied at concentrations of 50ng/ml,
5ng/ml, 500pg/ml, 50pg/ml and 5pg/ml to five different culture
flasks with one additional flask acting as control. Sanicle was
applied at concentrations of 100μg/ml, 10μg/ml, 1μg/ml,
100ng/ml and 10ng/ml with one additional flask as a control.
The cells were imaged over a period of 20 hours with or without
presence of TGF-β3 and Sanicle. The results indicated that
although there were no significant increases in the rate of wound
closure in relation to application of TGF-β3, there is an
indication that TGF-β3 may enhance model wound closure at
optimum working concentration between 5ng/ml and 50ng/ml.
However, the sanicle extract did not stimulate enhanced repair
of the model in vitro wound, but instead seemed to promote cell
death along the wound margin. These results indicate that
sanicle may be used in the care of wounds, but not as a growth
promoter, but because it acts as an antibiotic agent, and possibly
because it aids wound debridement.
Version
No full-text in the repository
Citation
Beggs, C.B., Denyer, M.C.T., Lemmerz, A., Sefat, F., Wright, C. and Youseffi, M. (2010) The effect of transforming growth factor beta (TGF-β3) and sanicle on wound healing. Proceedings of the World Congress on Engineering, WCE 2010, June 30-July 2, London, 2010.
Link to publisher’s version
Link to published version
Link to Version of Record
Type
Conference paper