Loading...
Effect of transforming growth factor-β2 on biological regulation of multilayer primary chondrocyte culture
Khaghani, Seyed A. Akbarova, G. Soon, C.F. Dilbazi, G.
Khaghani, Seyed A.
Akbarova, G.
Soon, C.F.
Dilbazi, G.
Publication Date
2018-12
End of Embargo
Supervisor
Rights
(c) 2018 The Authors. This is an Open Access article distributed under the Creative Commons CC-BY license (http://creativecommons.org/licenses/by/4.0/)
Peer-Reviewed
Yes
Open Access status
openAccess
Accepted for publication
15/10/2018
Institution
Department
Awarded
Embargo end date
Collections
Additional title
Abstract
Cytokines are extremely potent biomolecules that regulate cellular functions and play multiple roles in initiation and inhibition of disease. These highly specialised macromolecules are actively involved in control of cellular proliferation, apoptosis, cell migration and adhesion. This work, investigates the effect of transforming growth factor-beta2 (TGF-β2) on the biological regulation of chondrocyte and the repair of a created model wound on a multilayer culture system. Also the effect of this cytokine on cell length, proliferation, and cell adhesion has been investigated. Chondrocytes isolated from knee joint of rats and cultured at 4 layers. Each layer consisted of 2 × 105 cells/ml with and without TGF-β2. The expression of mRNA and protein levels of TGF-β receptors and Smad1, 3, 4, and 7 have been analysed by RT-PCR and western blot analysis. The effect of different supplementations in chondrocyte cell proliferation, cell length, adhesion, and wound repair was statistically analysed by One-way ANOVA test. Our results showed that the TGFβ2 regulates mRNA levels of its own receptors, and of Smad3 and Smad7. Also the TGF-β2 caused an increase in chondrocyte cell length, but decreased its proliferation rate and the wound healing process. TGF-β2 also decreased cell adhesion ability to the surface of the culture flask. Since, TGF-β2 increased the cell size, but showed negative effect on cell proliferation and adhesion of CHC, the effect of manipulated TGF-β2 with other growth factors and/or proteins needs to be investigated to finalize the utilization of this growth factor and design of scaffolding in treatment of different types of arthritis.
Version
Published version
Citation
Khaghani SA, Akbarova G, Soon CF et al (2018) Effect of transforming growth factor-β2 on biological regulation of multilayer primary chondrocyte culture. Cell and Tissue Banking. 19(4): 763-775.
Link to publisher’s version
Link to published version
Link to Version of Record
Type
Article