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Recent advances in the analysis of polysialic acid from complex biological systems
Guo, Xiaoxiao Elkashelf, Sara M. Patterson, Laurence H. Falconer, Robert A.
Guo, Xiaoxiao
Elkashelf, Sara M.
Patterson, Laurence H.
Falconer, Robert A.
Publication Date
2019-11-15
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Crown Copyright © 2019 Published by Elsevier Ltd. All rights reserved. Reproduced in accordance with the publisher's self-archiving policy. This manuscript version is made available under the CC-BY-NC-ND 4.0 license.
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Yes
Open Access status
openAccess
Accepted for publication
2019-07-29
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Abstract
Polysialic acid (polySia) is a unique, well-characterised carbohydrate polymer highly-expressed on the cell surface of neurons in the early stages of mammalian brain development. Post-embryogenesis, it is also re-expressed in a number of tumours of neuroendocrine origin. It plays important roles in modulating cell-cell, and cell-matrix adhesion and migration, tumour invasion and metastasis. Techniques for structural and quantitative characterisation of polySia from tumours and cancer cells are thus essential in exploring the relationship between polySia expression levels and structural and functional changes associated with cancer progression and metastasis. A variety of techniques have been developed to structurally and quantitatively analyse polySia in clinical tissues and other biological samples. In this review, analytical approaches used for the determination of polySia in biological matrices in the past 20 years are discussed, with a particular focus on chemical approaches, and quantitative analysis.
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Accepted manuscript
Citation
Guo X, Elkashef SM, Loadman PM et al (2019) Recent advances in the analysis of polysialic acid from complex biological systems. Carbohydrate Polymers. 224: 115145.
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Article